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Position: Home > Articles > Cloning and Sequence Analysis of Potato Virus Y pl Gene from Shanxi Province Journal of Henan Agricultural Sciences 2009,18 (5) 116-118

山西省马铃薯Y病毒pl基因的克隆与序列分析

作  者:
吴兴泉;刘晓磊;陈士华;曹成;陈琼珍;侯志鹏
单  位:
河南省谷物资源转化与利用省级重点实验室河南工业大学
关键词:
山西省;马铃薯Y病毒;pl基因
摘  要:
依据马铃薯Y病毒基因组序列,设计合成了1对引物,以从山西省获得的带毒马铃薯叶片总RNA为模板,通过RT-PCR扩增得到852 bp的基因片段并进行了序列测定。BLAST分析表明,该DNA序列与PVY N:O株系pl基因序列相似性最高可达99%。
译  名:
Cloning and Sequence Analysis of Potato Virus Y pl Gene from Shanxi Province
作  者:
WU Xing-quan,LIU Xiao-lei,CHEN Shi-hua,CAO Cheng,CHEN Qiong-zhen,HOU Zhi-peng(The Key Lab of Conversion and Utilization of Grain Resource of Henan Province,Henan University of Technology,Zhengzhou 450052,China)
关键词:
Shanxi province;Potato virus Y;pl gene
摘  要:
A pair of specific primers was designed based on the gene sequence of potato virus Y,which amplified a 852 bp DNA fragment from the total RNA of potato leaves infected by PVY collected from Shanxi province by RT-PCR.Sequence analysis showed that the DNA fragment amplified had 99% similarity with that of pl gene of PVY strain N:O.

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