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当前位置: 首页 > 文章 > 桃枝枯病病原鉴定 扬州大学学报(农业与生命科学版) 2013,34 (4) 98-102
Position: Home > Articles > Identification of the pathogen causing shoot blight of peach trees Journal of Yangzhou University(Agricultural and Life Science Edition) 2013,34 (4) 98-102

桃枝枯病病原鉴定

作  者：
纪兆林;戴慧俊;金唯新;宋宏峰;张慧琴;金建芳;熊彩珍;徐敬友
单  位：
嘉兴市南湖区林业与蚕桑站;浙江省农业科学院园艺研究所;嘉兴市南湖区凤桥镇农业服务中心;无锡市惠山区阳山镇农业技术综合服务站;扬州大学园艺与植物保护学院;江苏省农业科学院园艺研究所
关键词：
桃枝枯病;形态特征;致病性;ITS序列;桃拟茎点霉
摘  要：
为明确近年来新发现的严重危害桃树的桃枝枯病病原种类,采集浙江和江苏两地的桃枝枯病病枝,对病原进行分离和鉴定。通过对真菌分离物培养性状、分生孢子器和分生孢子形态观察,两地真菌分离物均为桃拟茎点霉(Phomopsis amygdali)。ITS1-5.8SrDNA-ITS2序列分析表明两地真菌分离物为同一种病原菌,与GenBank中桃拟茎点霉序列具有99%的相似性,其ITS序列已在GenBank中登录(登录号分别为JX081273和JX182974)。离体和活体致病性测定结果表明,分离菌株均能使桃健康新梢产生枝枯病症状,从接种发病部位再次分离到病原菌,完成了柯赫氏法则(Koch’postulates)验证。结合其形态特征、ITS序列和致病性测定结果,浙江和江苏桃枝枯病病原确定为桃拟茎点霉。
译  名：
Identification of the pathogen causing shoot blight of peach trees
作  者：
JI Zhaolin;DAI Huijun;JIN Weixin;SONG Hongfeng;ZHANG Huiqin;JIN Jianfang;XIONG Caizhen;XU Jingyou;Coll of Hort and Plant Prot,Yangzhou Univ;Agric Tech Int Serv Sta of Yangshan Town,Huishan Dist,Wuxi City;Inst of Hort,Jiangsu Acad of Agric Sci;Inst of Hort,Zhejiang Acad of Agric Sci;Agric Serv Cen of Fengqiao Town;For and Seric Sta of Nanhu Dist;
关键词：
peach shoot blight;;morphological characteristics;;pathogenicity test;;ITS sequence;;Phomopsis amygdali
摘  要：
Peach shoot blight is an increasingly serious and new disease in Zhejiang and Jiangsu province peach growing areas in recent years.The pathogen was isolated from peach twigs with constriction canker from peach orchards in Jiaxing,Zhejiang province and Wuxi,Jiangsu province,respectively.Examination of the strains by morphological and cultural characteristics showed that the fungus was Phomopsis amygdali.The identification was verified by ITS analysis of rDNA.The sequences(GenBank accession Nos.JX081273and JX182974)shared 99.6%identity with each other.A comparison of these two sequences with those in GenBank showed that the sequences had the highest nucleotide similarity(99%)with P.amygdali isolate FAU1052from peach in the southeastern United States(accession No.AF102998). Pathogenicity tests in vivo and in vitro indicated that the isolates caused similar reddish brown around twigs as observed in the field.The pathogen was reisolated from inoculated plants,confirming Koch's postulates.Based on the pathogenicity,morphological characteristics and rDNA molecular analysis,the pathogen of peach shoot blight was finally identified as P.amygdali.

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