Cloning, muti-tissues qPCR expression and functional bioinformatics analysis of Banna mini-pig inbred line gene RNF4

ZHANG Xia;ZENG Ribin;HUO Jinlong;WANG Pei;CHEN Yuanyuan;WANG Shuyan;Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province, Yunnan Agricultural University;School of Animal Science and Technology, Yunnan Agricultural University;

RING finger;;Banna mini-pig inbred line(BMI);;tissue expression;;bioinformatics

RNF4 gene plays a regulater of sperm cell differentiation and proliferation process. The RNF4 m RNA sequence of pig and related species from Gen Bank was used as reference sequences, We designed specific primers and amplified Banna mini-pig inbred line(BMI) RNF4 gene. q PCR was applied to analysis the m RNA expression profiles of 15 important tissues. Protein sequence was used to carry out functional bioinformatics analysis and construct phylogenetic tree. A coding sequence of 573 bp(Gen Bank accession number: KU705638, the corresponding amino acid sequence accession number: AOC89056) of BMI RNF4 was obtained, which encodes a protein of 190 amino acids, protein molecular weight 21.43 ku,and isoelectric point 6.95. Fluorescence quantitative expression indicated that RNF4 gene expressed highly in the antiprostate, testis, liver, lung, and low expression in the other 11 tissues. Functional bioinformatics analysis indicated that RNF4 protein contained one conserved domains, no transmembrane region, no signal peptide sequences; its N-terminal and C-terminal were hydrophobic; it has four kinds functional active sites and a percentage 94.1 to located in cell nucleus. Phylogenetic analysis demonstrated that BMI had the closest relationship with dog. The results of the study will lay a foundation for further study of the gene about its functions in pig sperm cell differentiation and proliferation.