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Position: Home > Articles > Induction and Propagation of Hybrid Protocorm Like-body of Crosses between Cymbidium sinense and Cymbidium hybridium Acta Horticulturae Sinica 2004 (5) 688-690

墨兰与大花蕙兰种间杂种原球茎的诱导及增殖研究

作  者:
朱根发;陈明莉;罗智伟;罗思琼;吕复兵;王碧青
单  位:
广东省农业科学院花卉研究所
关键词:
大花蕙兰;墨兰;杂交;原球茎;增殖
摘  要:
利用墨兰与大花蕙兰进行种间杂交 ,成功获得了 5个组合的杂种原球茎和植株。墨兰×大花蕙兰及大花蕙兰×墨兰 ,均以原球茎方式萌发。以墨兰×大花蕙兰的杂种原球茎为材料 ,通过L9(34)正交设计研究了NH4NO3 、KH2 PO4、 6 BA和NAA等因素对杂种原球茎增殖的影响 ,发现NAA对原球茎的增殖影响不大 ,NH4NO3 16 5 0mg·L-1(MS正常浓度水平 ) ,提高KH2 PO4浓度 (170~ 85 0mg·L-1)和 6 BA浓度 (1~ 10mg·L-1)有利于原球茎的增殖。 9个培养基中以MS +NH4NO3 16 5 0mg·L-1+KH2 PO4340mg·L-1+6 BA 10mg·L-1效果最好。
译  名:
Induction and Propagation of Hybrid Protocorm Like-body of Crosses between Cymbidium sinense and Cymbidium hybridium
作  者:
Zhu Genfa,Chen Mingli, Luo Zhiwei, Luo Siqiong,Lü Fubing,and Wang Biqing (Floricultural Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)
关键词:
Cymbidium sinense;Cymbidium hybridium;Hybrid PLB; Propagation$$$$
摘  要:
Hybrid plants were obtained from 5 crosses between Cymbidium sinense and Cymbidium hybridium.The genotype of germination by protocorm like-body(PLB)of C.hybridium is controlled by dominant gene(s).Because all of these hybrid embryos,whatever C. sinense×C.hybridium or C.hybridium×C.sinense,are germinated by PLB,which is the same as C.hybridium.Four factors of NH_4NO_3、KH_2PO_4、6-BA and NAA with three different concentration(L_9)were designed to study the effects on induction and propagation of PLB of C.‘Jin Zui ’×C.‘Forgotten Fruits’.Analysis of variance shows: The different concentration among the factors which are NH_4NO_3、KH_2PO_4 and 6-BA influences the induction and propagation of PLB directly, There is no difference with different concentration of NAA. NH_4NO_3 1650 mg·L -1(MS normal levels), higher concentration of KH_2PO_4(170-850 mg·L -1)and BA densities(1-10 mg·L -1)was favorable to propagation of PLB. There are extremely remarkable differences among 9 media, the best is C_5 medium with NH_4NO_3 1650 mg·L -1,KH_2PO_4 340 mg·L -1and 6-BA 10 mg·L -1.

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