摘 要:
自然界中存在20种基本氨基酸,除甘氨酸外都有两种互成镜像的对映体D-型和L-型。长期以来,人们认为D-氨基酸仅存在于微生物中,参与构成细胞壁肽聚糖层。但随着分析方法的发展,人们相继在多种生物体中(包括人体)发现了多种D-氨基酸,D-氨基酸才引起人们的高度重视,并意识到它们在医药、农药和食品等的组成中起着重要作用。D-氨基酸检测方法还需要不断发展与完善,以便更好地分析和认识D-氨基酸的重要作用。主要介绍酶法、气相层析法、高效液相法及生物传感器等检测方法。
译 名:
Research Progress on the Detection Methods of D-amino Acids
作 者:
Li Huixin Ju Jiansong Zhang Linlin Xu Shujing(College of Life Science,Hebei Normal University,Shijiazhuang 050024)
关键词:
D-amino acid Detection HPLC Biosensor
摘 要:
As we all know there are 20 different kinds of basic amino acids in nature.In addition to glycine,each amino acid has two enantiomers D-and L-type.D-amino acids have long been considered to be unique to microorganisms,which utilize some D-amino acids to construct the peptidoglycan layer of their cell wall.However,several D-amino acids have been detected in many organisms including mammals because of the advance of analytical techniques in the last two decades.It was realized that D-amino acids might play significant roles in living things and as the important component of medicine,pesticide and food.In order to get a better analyzing and understanding the important role of D-amino acids,the analytical methods for D-amino acids detection are constantly developing.Herein,several methods include enzymatic method,Gas chromatography,HPLC and biosensor assay were introduced.