作 者：
杨会成;郑斌;廖妙飞;周宇芳;马华威;相兴伟;李八方
单 位：
中国海洋大学食品科学与工程学院;浙江省海洋开发研究院
关键词：
鮟鱇鱼;胶原蛋白肽;氨基酸;自由基;Hyp
摘 要：
研究提取鮟鱇(Lophius litulon)胶原蛋白肽的最佳制备工艺,测定鱼皮基本成分和多肽的氨基酸组成,探讨鮟鱇多肽组分体外自由基清除效果。结果表明,鱼皮中粗蛋白占28.1%;胃蛋白酶添加量1%,料液比1∶20,酶解时间6h,酶解温度5℃时,羟脯氨酸提取率为11.67%,鮟鱇胶原蛋白肽提取效果最佳;鮟鱇鱼皮胶原蛋白肽在232nm处有紫外吸收峰,胶原肽中Gly(甘氨酸)占36.61%,Pro(脯氨酸)和Hyp(羟脯氨酸)总含量占16.86%,Phe(苯丙氨酸)、Cys(半胱氨酸)、His(组氨酸)三者含量均低于1.1%;鮟鱇鱼皮胶原蛋白肽具有较强的还原能力且对O-2·(超氧阴离子自由基)、DPPH·(二苯基苦酰肼基自由基)、·OH(羟自由基)具有一定的清除效果,清除能力与胶原蛋白肽的浓度呈正相关,浓度为10mg/mL时,对·OH、DPPH·、O-2·自由基的清除率和Fe3+还原力分别为70.48%、68.78%、48.33%和0.676。
译 名：
Study on optimal preparation process and free radical scavenging activity of collagen peptides from Lophius litulon skin
作 者：
YANG Hui-cheng;ZHENG Bin;LIAO Miao-fei;ZHOU Yu-fang;MA Hua-wei;XIANG Xing-wei;LI Ba-fang;College of Food Science and Engineering,Ocean University of China;Zhejiang Marine Development Research Institute;
关键词：
Lophius litulon;;collagen peptides;;amino acid;;free radicals;;Hyp
摘 要：
Collagen peptides from Lophius litulon skin had been extracted by using pepsin with orthogonal design. The composition of Lophius litulon skin,amino acid composition and UV absorption of the collagen peptides were analyzed. And four representive free radical systems were selected to evaluate the free radicals scavenging effection of Lophius litulon skin collagen peptides in vitro. The results showed that the crude protein in skin was 28.1%,the optimal preparation process of collagen peptides from Lophius litulon skin was under this condition,that the pepsin addition was 1%,the ratio between fish skin and solvent was 1∶20,extracting for6 h at the temperature of 5℃,and the Hyp yield of extraction was 11.67%. Ultraviolet Spectrum and amino acid analysis on collagen peptides showed that the maximum absorption peak was being 232 nm and the proportion of Gly was 36.61%,Pro with Hyp was 16.86%,the proportion of Phe,Cys and His were less than 1.1% respectivly.The collagen peptides showed a certain capacity on free radical scavenging and reducibility,which was positive correlation with concentration of collagen peptides. The free radical scavenging rate of ·O,DPPH·,O-2·and reducibility of Fe3 +respectivly were 70.48%,68.78%,48.33% and 0.676 at the collagen peptides concentration of 10mg/mL.
