Position: Home > Articles > The Effect of Trichostatin A on Goat Cloned Embryo Development Following Treatment of Donor Cells or Reconstructed Embryos
Chinese Journal of Animal and Veterinary Sciences
2009,40
(6)
824-829
TSA处理供体细胞或重构胚对山羊克隆胚胎发育的影响
作 者：
刘凤军;杨自军;张玉玲;赵树科;汪纪仓
单 位：
河南科技大学动物科技学院
关键词：
曲古菌素A;核移植;DNA甲基化;组蛋白去乙酰化;山羊
摘 要：
本研究探讨了TSA处理供体细胞或重构胚对山羊克隆胚胎发育的影响,不处理组设为对照组。选择经5、25、50、75、100 nmol.L-1TSA处理24 h的山羊胎儿成纤维细胞作为供体细胞进行核移植,结果50和75nmol.L-1组的囊胚率显著高于对照组(27.34%、26.89%vs 16.18%,P<0.05);用5、25、50、75或100 nmol.L-1TSA处理重构胚10 h,结果5、25、507、5 nmol.L-1组的囊胚率均有所提高,其中50 nmol.L-1组显著高于对照组(27.34%vs 16.53%,P<0.05)。结果表明,TSA处理供体细胞和重构胚均能显著提高山羊克隆胚囊胚率,说明TSA可能降低了核移植后供体细胞组蛋白去乙酰化水平和DNA甲基化水平,因而提高了克隆胚的发育率。
译 名：
The Effect of Trichostatin A on Goat Cloned Embryo Development Following Treatment of Donor Cells or Reconstructed Embryos
作 者：
LIU Feng-jun*,YANG Zi-jun,ZHANG Yu-ling,ZHAO Shu-ke,WANG Ji-cang(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China)
关键词：
trichostatin A;nuclear transfer;DNA methylation;histone deacetylaiton;goat
摘 要：
To investigate the effect of TSA on goat somatic cell nuclear transfer(SCNT)-embryo development,we treated goat fetal fibroblasts for 24 h prior to nuclear transfer or reconstructed embryos for 10 h following oocyte activation with TSA.50 and 75 nmol·L-1 TSA treatment of donor cells increased blastocyst development compared to control(27.34%,26.89% vs 16.18%,P<0.05).Development to blastocysts by embryo cloned from 5-75 nmol·L-1 TSA treatment of reconstructed embryos was higher than that in embryos cloned from untreated control,moreover blastocyst rate of 50 nmol·L-1 group was significantly higher than that of control(27.34% vs 16.53%,P<0.05).These results indicated that TSA-treatment can dramatically improve subsequent in vitro development of cloned embryos,and could be partially decrease the level of DNA methylation and histone deacetylation of donor cells.
导出引用
计量
文章访问数: 4
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
