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当前位置: 首页 > 文章 > 根瘤农杆菌介导ACO基因转化延长石竹花期的研究 华中农业大学学报 2015,34 (6) 21-26
Position: Home > Articles > Extending the flowering period of Dianthus chinensis with Agrobacterium-mediated ACO gene transformation Journal of Huazhong Agricultural University 2015,34 (6) 21-26

根瘤农杆菌介导ACO基因转化延长石竹花期的研究

作  者：
吴晓庆;谭华山;张静静;包满珠;傅小鹏
单  位：
华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室
关键词：
石竹;农杆菌介导的遗传转化;ACO基因;花期
摘  要：
以石竹(Dianthus chinensis)叶片为外植体,通过根癌农杆菌介导的遗传转化体系,转ACO基因获得花期延长的石竹材料。结果表明:以MS为基本培养基,BA和NAA质量浓度分别为1.0、0.3 mg/L时,供试材料H58II、X82I和H68Ⅲ分化率较高,分别为56.9%、31.7%、93.2%;遗传转化筛选过程中,Kan、Hyg、Cef的最适筛选质量浓度分别为40、4和400mg/L;染色检测表明,转化成功的叶片或植株染色后呈现蓝色;PCR检测转ACO基因植株,阳性率达到89%。田间观察转ACO基因植株的瓶插花期和单朵花期可达到12d,比未转ACO基因植株的花期(6d)增加1倍;转ACO基因植株的盆栽群体花期可达到45d,比未转ACO基因植株的盆栽群体花期(30d左右)延长15d。
译  名：
Extending the flowering period of Dianthus chinensis with Agrobacterium-mediated ACO gene transformation
作  者：
WU Xiao-qing;TAN Hua-shan;ZHANG Jing-jing;BAO Man-zhu;FU Xiao-peng;Key Laboratory of Horticultural Plant Biology,Ministry of Education/College of Horticulture and Forestry Sciences,Huazhong Agricultural University;
关键词：
Dianthus chinensis;;Agrobacterium-mediated transformation;;ACO gene;;flowering period
摘  要：
The leaves of Dianthus chinensis were used to transform ACO gene with Agrobaceriumtumefaciens-mediated system to obtain the new Dianthus with extending flowering period.Results showed that H58Ⅱ,X82 Iand H68Ⅲ had high differentiation rates of 56.9%,31.7% and 93.2% when the concentrations of BA and NAA in the MS medium were 1.0mg/L and 0.3 mg/L.The optimal concentration of kanamycin,hygromycin and cephalosporin was 40.0mg/L,4.0mg/L and 400.0mg/L in the selective medium during selective culture of transformation.Transformed leaves and plants presented blue color after being stained.Molecular test showed that the positive rate of transgenic plants was 89%.The vase life for a single flower of plants transformed with ACO gene was 12 days compared with the wild plants flowers of 6days.The population flowering period of transformed plants in pots was 45 days while that of wild plants was 30 days.

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