醋酸纤维素-聚丙烯复合膜固定化转谷氨酰胺酶的研究
作 者：
时敏;王雪;马丽娜;于殿宇
单 位：
东北农业大学食品学院
关键词：
醋酸纤维素-聚丙烯复合膜;转谷氨酰胺酶;酶活力;固定化
摘 要：
通过醋酸纤维素-聚丙烯复合膜对转谷氨酰胺酶进行固定,以酶活力为指标,进行单因素试验,考察酶液质量浓度、吸附时间、戊二醛添加量、戊二醛交联时间对转谷氨酰胺酶活力的影响,确定最佳固定化条件为:酶液质量浓度15mg/mL、吸附时间3h、戊二醛添加量0.3g/100mL、戊二醛交联时间4h.在最佳固定化条件下固定的酶活力可达16.1U/g膜.对固定化酶和游离酶的酶学性质进行比较,得出游离酶最适温度为35~40℃,而固定化酶膜最适温度为45~50℃;游离酶最适pH值为6~7,固定化酶膜最适pH值为5~6,固定化酶比游离酶向酸性偏移.
单 位：
School of Food Science and Technology,Northeast Agricultural University,Harbin 150030,China
关键词：
cellulose acetate-polypropylene composite membrane%transglutaminase%enzyme activity%immobilization
摘 要：
The transglutaminase was immobilized on the cellulose acetate-polypropylene composite membrane in this study.The activity of the enzyme was used as the index.Based on the single-factor tests,the effects of enzyme addition amount,absorption time,glutaraldehyde concentration and glutaraldehyde cross-linking time on the activity of enzyme were studied.The optimal immobilization conditions required enzyme concentration of 15 mg/mL,adsorption time of 3 h,glutaraldehyde concentration of 0.3 g/100 mL,glutaraldehyde cross-linking time of 4 h.The activity of immobilized enzyme under the optimal immobilization conditions was up to 16.1 U/g.The optimal temperature for the free enzyme was 35-40 ℃,compared to 45-50 ℃ for the immobilized enzyme.The optimal pH for the free enzyme was 6-7,compared to pH 5-6 for the immobilized enzyme.Compared with the free enzyme,the immobilized enzyme was shifted to the acidity side.
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