中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 右美托咪定对腹腔镜气腹致大鼠肠损伤的影响 中国兽医学报 2023,43 (10) 2109-2115
Position: Home > Articles > Effect of dexmedetomidine on intestinal injury in rats induced by laparoscopic pneumoperitoneum Chinese Journal of Veterinary Science 2023,43 (10) 2109-2115

右美托咪定对腹腔镜气腹致大鼠肠损伤的影响

作  者：
张暖暖;宁夏青;艾丽平;孙晨;张士霞
单  位：
关键词：
右美托咪定;气腹;小肠;炎性反应;氧化应激;大鼠
摘  要：
旨在探讨右美托咪定对气腹所致肠损伤的保护作用.将18只SD大鼠分为3组(6只/组):假手术组(Sham)、CO2气腹组(CO2)、右美托咪定组(DEX).CO2组和DEX组在2 kPa(15 mmHg)气腹压力下维持90 min建立气腹模型;DEX组在气腹前30 min腹腔注射50 μg/kg右美托咪定;Sham组不通气腹,其余操作同CO2组.气腹结束6 h收集小肠样本.通过HE染色观察小肠组织病理形态学变化;采用ELISA法检测IL-6、IL-1β、TNF-α水平;化学发光法检测MDA、GSH含量和MPO活性;采用RT-qPCR检测紧密连接蛋白ZO-1、Occludin和Claudin-1的mRNA表达;Western blot 检测 HO-1、Nrf2、IKBα、NF-κB 蛋白表达.结果显示:与 Sham 组相比,C02组 IL-6、IL-1β、TNF-α、MDA含量和MPO活力均极显著升高(P<0.01),GSH含量极显著降低(P<0.01),ZO-1、Occludin、Claudin-1的mRNA相对表达量均极显著降低(P<0.01),HO-1、Nrf2、IKBα和NF-κB的蛋白表达极显著升高(P<0.01);与CO2组相比,DEX组IL-6、IL-1β、TNF-α、MDA含量和MPO活力均极显著下降(P<0.01),GSH含量极显著升高(P<0.01),ZO-1、Occludin、Claudin-1的mRNA相对表达量极显著升高(P<0.01),HO-1、Nrf2蛋白表达极显著升高(P<0.01),IKBα蛋白表达极显著下降(P<0.01),NF-κB蛋白表达显著下降(P<0.05).上述结果表明,右美托咪定可以通过抗氧化、降低炎性反应等减轻腹腔镜气腹所致肠损伤.
译  名：
Effect of dexmedetomidine on intestinal injury in rats induced by laparoscopic pneumoperitoneum
关键词：
dexmedetomidine%pneumoperitoneum%small intestine%inflammatory response%oxidative stress%rat
摘  要：
The aim of this experiment was to investigate the protective effect of dexmedetomidine on pneumoperitoneum-induced intestinal injury.In this experiment,18 SD rats were divided into 3 groups(6 rats/group):sham-operated group(Sham),CO2 pneumoperitoneum group,and dexme-detomidine group(DEX).The CO2and DEX groups were maintained at 15 mmHg pneumoperito-neum pressure for 90 min to establish the pneumoperitoneum model;the DEX group was injected with 50 μg/kg dexmedetomidine intraperitoneally 30 min before pneumoperitoneum;the Sham group had no pneumoperitoneum and the rest of the operation was the same as the CO2 group.The small intestine samples were collected 6 h after the end of pneumoperitoneum.The histomorpho-logical changes of small intestine were observed by HE staining;IL-6,IL-1β and TNF-α levels were detected by ELISA;MDA,GSH content and MPO activity were detected by chemiluminescence;mRNA expression of tight junction proteins ZO-1,Occludin and Claudin-1 were detected by RT-qPCR;HO-1,Nrf2,IKBα and NF-κB protein expression were detected by Western blot.The results showed that compared with the Sham group,the CO2 group showed highly significant increases in IL-6,IL-1β,TNF-α,MDA content and MPO activity(P<0.01),highly significant decreases in GSH content(P<0.01),highly significant decreases in the relative mRNA expressions of ZO-1,Occludin and Claudin-1(P<0.01),and highly significant increases in HO-1,Nrf2,IKBα and NF-κB protein expressions were highly significantly increased(P<0.01).Compared with the CO2 group,the DEX group showed a highly significant decrease in IL-6,IL-1β,TNF-α,MDA content and MPO activity(P<0.01),a highly significant increase in GSH content(P<0.01),a highly sig-nificant increase in the relative mRNA expression of ZO-1,Occludin and Claudin-1(P<0.01),and a highly significant increase in HO-1,Nrf2 protein expression was highly significantly elevated(P<0.01),IKBα protein expression was highly significantly decreased(P<0.01),and NF-κB pro-tein expression was significantly decreased(P<0.05).These results suggest that dexmedetomidine can reduce intestinal injury due to laparoscopic pneumoperitoneum by antioxidant and reducing in-flammatory response.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46