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当前位置: 首页 > 文章 > 自制简易胚胎冷冻器冷冻奶牛胚胎试验 西北农林科技大学学报(自然科学版) 1993 (S2) 69-72
Position: Home > Articles > Experiment of Freezing Bovine Embryos with Self-made Simple Freezer Journal of Northwest A & F University(Natural Science Edition) 1993 (S2) 69-72

自制简易胚胎冷冻器冷冻奶牛胚胎试验

作  者：
窦忠英;王新庄;樊敬庄;高志敏;徐照学;国永周;韩晓良;李继合;刘恩耀;张强;王效涵;薛帅岐
单  位：
西北农业大学奶牛胚胎移植试验站;西北农业大学奶牛胚胎移植试验站,西北农业大学奶牛胚胎移植试验站,西北农业大学奶牛胚胎移植试验站,西北农业大学奶牛胚胎移植试验站,西北农业大学奶牛胚胎移植试验站,西北农业大学奶牛胚胎移植试验站,西北农业大学奶牛胚胎移植试验站,西安市红庙坡奶牛场,西安市红庙坡奶牛场,西安市红庙坡奶牛场,西安市未央区畜牧兽医工作站,武功县武功镇畜牧兽医工作站 陕西杨陵 712100,陕西杨陵 712100,陕西杨陵 712100,陕西杨陵 712100,陕西杨陵 712100,陕西杨陵 712100,陕西杨陵 712100
关键词：
冷冻胚胎;妊娠率;奶牛
摘  要：
用FSH+PGF_(2)超排黑白花奶牛,以非手术方法采得7日龄早期胚胎。应用含10％甘油卵细胞培养液作为胚胎冷冻保护液,采用自制简易胚胎冷冻器冷冻,在-196℃液氮中保存。冻胚在35℃水浴中解冻,应用92.1g/L甘油+171.2g/L蔗糖PBSS,46g/L甘油+171.2g/L蔗糖PBSS和171.2g/L蔗糖PBSS三步脱去甘油,用含20％犊牛血清PBS将胚胎清洗2遍后装管移植。共解冻18枝冻胚,16枚可用。可用胚率为84.2％(16/18)。采用非手术方法移植16头受体牛,5头妊娠产犊,移植妊娠率为31.5％(5/16)。
译  名：
Experiment of Freezing Bovine Embryos with Self-made Simple Freezer
作  者：
Dou Zhongying Wang Xinzhuang Fan Jingzhuang Gao Zhimin Xu Zhaoxue Guo Yungzhou Han Xiaoliang(Experimental Station of Dairy Cow Embryo Transfer Northwestern Agricultural University,Wangling,Shaanxi,China 712100)Li Jihe Liu Enyao Zhang Qiang(Hongmiaopo Diary Cattle Farm, Xian, Shaanxi)Wang Xiaohan(Weiyang Animal.Husbandry and Veterinary Station,Xian,Shaanxi)Xue Shuaqi(.Animal Husbandry and Veterinary Station of Wugong Town,Wugong,Shaanxi')
关键词：
embryo freezing, pregnancy rate, dairy cattle
摘  要：
Donor Holsteins were superovulated with Fsh+PGF2, and flushed non-surgically at day 7. Cryoprotectant was cocyte culture medium containing 10% glycerol. Fmbryo freezing was accomplished by means of self-made freezier. The embryos were stored in liquid nitrogen at -196℃. Frozen embryos were thawed in a 35'C water bath, and the cryoprotectant was removed by three steps of 92. 19 g/L glycerol+ 171. 2 g/L sucrose in PBSS,46 g/L glycerol + 171. 2 g/L sucrose in PBSS. and 171. 2 g/L sucrose in PBSS. Embryos were washed twice in 20% PBS and transferred non-surgically into the uterus of recipients. Eighteen frozen embryos were thawed, sixteen of them could be used. The rate of available embryos was 84. 2% (16/18). Available embryos were transferred non -surgically to 16 recipients. Five of them be came pregnant. The transplant pregnancy rate was 31. 3% (5/16).

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