Position: Home > Articles > Determination of Polysaccharide in Sporophyll of Undaria pinnatifida Suringar
FOOD SCIENCE
2005,26
(10)
184-186
裙带菜孢子叶多糖含量的测定
作 者：
朱良;王一飞;朱艳梅
单 位：
暨南大学生物医药研究开发基地
关键词：
裙带菜孢子;多糖;苯酚-硫酸法;含量测定
摘 要：
用苯酚-硫酸比色法测定了裙带菜孢子叶中多糖的含量。测定波长489nm,多糖换算因子f=2.51;在5～80μg/ml范围内吸收度与被测物含量呈良好的线性关系,r=0.9998;多糖的平均回收率为98.57%,RSD为2.31%(n=5)。本法简便、准确、重现性好,可作为裙带菜孢子叶中多糖的含量测定方法。
译 名：
Determination of Polysaccharide in Sporophyll of Undaria pinnatifida Suringar
作 者：
ZHU Liang,WANG Yi-fei,ZHU Yan-mei (Biomedical Research and Development Center of Jinan University, Guangzhou 510632, China)
关键词：
sporophyll of Undaria pinnatifida Suringar;polysaccharide;anthrone-sulfuric acid determination
摘 要：
The contents of polysaccharide in Sporophyll of Undaria pinnatifida Suringar. were assayed by phenol-H2SO4 colorimetry. The results were wavelength for measurement was 489nm and converting factor of polysaccharide 2.51; good linearity ranged from 5～80μg/ml,r=0.9998 and average recovery rate of polysaccharide 98.57% with 2.31% of RSD(n=5).The method used in this paper was simple, accurate and stable. It could be used for determination of the polysaccharide in leaves of sporophyll of Undaria pinnatifida Suringar.
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