Position: Home > Articles > Isolation and Identification of Pathogenic Bacterium Citrobacter Freundii from Blacktip Reef Shark Carcharhinus melanopterus
Transactions of Oceanology and Limnology
2015
(4)
83-88
乌翅真鲨弗氏柠檬酸杆菌的分离与鉴定
作 者：
卢君辉;孙佳佳;董世龙;任硕
单 位：
北京自然博物馆
关键词：
乌翅真鲨;弗氏柠檬酸杆菌;分离鉴定;药敏试验
摘 要：
为确定引起乌翅真鲨(Carcharhinus melanopterus)患病的病原种类及耐药情况,用常规方法从患病乌翅真鲨内脏取样分离得到的1株优势菌,通过回归感染试验确定该菌株的致病性,通过经典生理生化分析、API 20E细菌快速鉴定系统、16SrDNA序列分析对其进行鉴定,确定该株致病菌为弗氏柠檬酸杆菌(Citrobacter freundii)。药敏试验结果表明:该菌株对左氧氟沙星、头孢曲松钠、四环素、链霉素和庆大霉素等敏感。
译 名：
Isolation and Identification of Pathogenic Bacterium Citrobacter Freundii from Blacktip Reef Shark Carcharhinus melanopterus
作 者：
LU Junhui;SUN Jiajia;DONG Shilong;REN Shuo;Beijing Museum of Natural History;
关键词：
Blacktip reef shark;;carcharhinus melanopteru;;citrobacter freundii;;isolation and identification;;antibiotic sensitivity
摘 要：
This experiment was conducted to clarify pathogenic species and its drug resistance of isolated from the diseased blacktip reef shark Carcharhinus melanopterus.A dominant bacterial strain was isolated from the internal organ of the diseased shark.Pathogenicity of the isolated strain was determined by artificial infection.Bacterium was identified by traditional physiological and biochemical characteristics,API identification system and analysis of 16 SrDNA.The strain was identified as Citrobacter freundii.The susceptibility test to antibiotics demonstrated that the bacterial strain was susceptible to levofloxacin,ceftriaxone sodium,tetracycline,streptomycin and gentamicin.
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