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Position: Home > Articles > Expression Analysis of WRKY Family in Response to Salt Stress in Malus zumi Mats Acta Horticulturae Sinica 2010,37 (8) 1213-1219

珠美海棠MzWRKY基因家族盐胁迫应答模式研究

作  者:
蒋阿维;张素维;孙杨吾;褚欣然;赵宇;许雪峰;孔瑾
单  位:
北京市果树逆境生理与分子生物学实验室;中国农业大学农学与生物技术学院
关键词:
珠美海棠;WRKY转录因子;盐胁迫;半定量RT-PCR
摘  要:
对苹果属植物现有的58个WRKY的EST序列进行分析,得到了37条uniESTs。珠美海棠(Malus zumi Mats)是耐盐的优良苹果砧木,能在土壤全盐含量为0.6%的盐碱地上存活。根据已知的37条uniESTs序列,通过半定量RT-PCR研究了珠美海棠中MzWRKY基因家族的盐应答模式。28个MzWRKY基因能检测到RT-PCR的产物,其中21个基因受盐诱导,1个受盐抑制。根据MzWRKY基因盐应答模式可将这些基因分为两组,基因表达模式的差异说明MzWRKY在珠美海棠的盐应答中角色的多样性。
译  名:
Expression Analysis of WRKY Family in Response to Salt Stress in Malus zumi Mats
作  者:
JIANG A-wei,ZHANG Su-wei,SUN Yang-wu,CHU Xin-ran,ZHAO Yu,XU Xue-feng,and KONG Jin (Laboratory of Stress Physiology and Molecular Biology for Fruit Tree,College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China)
关键词:
Malus zumi Mats;WRKY transcription factor;salt stress;semi-quantitative RT-PCR
摘  要:
Sequence assembly from 58 Malus WRKY ESTs resulted in 37 uniESTs.Malus zumi Mats is a salt-tolerant rootstock of apple,which could survive in 0.6% total salt content of land.Primers were designed in M.zumi Mats according to the 37 uniESTs,semi-quantitative RT-PCR was applied to reveal the expression patterns of MzWRKY gene family in response to salt stress.RT-PCR products of 28 MzWRKY genes were detectable,21 MzWRKYs were up-regulated and 1 MzWRKY was down regulated under salt stress.According to the peak hour,the MzWRKYs were classified into 2 groups.The different expression patterns of MzWRKY genes suggested that WRKY proteins play various roles in response to salt stress in M.zumi Mats.

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