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Position: Home > Articles > EMS mutagenesis and ALS- inhibitor herbicide- resistant mutants of Brassica napus L. Chinese Journal of Oil Crop Sciences 2014,36 (1) 25-31

甘蓝型油菜EMS突变体库构建及抗除草剂突变体筛选

作  者:
曲高平;孙妍妍;庞红喜;吴强;王发禄;胡胜武
单  位:
青海省农业技术推广总站;旱区作物逆境生物学国家重点实验室;青海省尖扎县种子管理站
关键词:
甘蓝型油菜;EMS;诱变;苯磺隆;抗除草剂突变体
摘  要:
为了利用诱变技术快速创造变异类型丰富的油菜新材料用于油菜遗传改良,分别用0.4%、0.8%、1.0%和1.2%的EMS(甲基磺酸乙酯)溶液对甘蓝型油菜中双9号种子进行诱变处理。结果发现:(1)1.0%的EMS溶液是处理中双9号干种子的最适浓度,4种不同浓度处理M2表型变异率依次为11.26%、14.82%、27.19%和12.38%;(2)EMS诱变具有器官特异性;(3)M2突变体库中筛选到3株苯磺隆抗性突变体,突变率约为10-4。现已创建了包括子叶、叶片、花器、株型、角果等多器官变异类型的突变体库,为油菜遗传研究提供了丰富的种质资源。
译  名:
EMS mutagenesis and ALS- inhibitor herbicide- resistant mutants of Brassica napus L.
作  者:
QU Gao-ping;SUN Yan-yan;PANG Hong-xi;WU Qiang;WANG Fa-lu;HU Sheng-wu;State Key Laboratory of Crop Stress Biology for Arid Area;College of Agronomy,Northwest A& F University;Agricultural Technology Extension Station of Qinghai Province;Jianza County Seed Management Station of Qinghai Province;
关键词:
Brassica napus L.;;Ethyl methansulfonate;;Mutation;;Tribenuron-methyl;;Herbicide-resistant mutant
摘  要:
To enhance rapeseed germplasm and improve rapeseed variety with resistance to tribenuron- methyl,seeds of Zhongshuang 9( Brassica napus) were treated with 0. 4%,0. 8%,1. 0% and 1. 2% of EMS( ethyl methansulfonate) solution respectively. Results showed that( 1) 1. 0% of EMS was optional for mutagenesis and the phenotype variation in four M2populations were 11. 26%,14. 82%,27. 19% and 12. 38% respectively.( 2) Different concentrations of EMS had different effects on organs.( 3) Three mutants with resistance to tribenuron-methyl were with mutation frequency about 0. 000 1. The M2mutant library,including mutants of cotyledons,leaves,flowers,plant- types,siliques,was available for genetic studies and breeding in rapeseed.

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