中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 芹菜细菌性软腐病病原的分离与鉴定 植物病理学报 2016,46 (3) 304-312
Position: Home > Articles > Isolation and identification of the pathogen causing celery bacterial soft rot Acta Phytopathologica Sinica 2016,46 (3) 304-312

芹菜细菌性软腐病病原的分离与鉴定

作  者：
晋知文;宋加伟;谢学文;柴阿丽;石延霞;李宝聚
单  位：
中国农业科学院蔬菜花卉研究所
关键词：
芹菜;细菌性软腐病;胡萝卜软腐果胶杆菌;多基因序列分析(MLSA);鉴定
摘  要：
由植物病原细菌引起的芹菜软腐病在北京地区普遍发生,其中以顺义及通州区县较为严重。自发病芹菜茎段中分离细菌,通过接种芹菜进行致病性测定,确定了54个致病菌株。虽然菌株间致病力有一定的差异,但大多数菌株对芹菜致病力强。通过培养性状和菌体形态观察、生理生化反应和Biolog测定,结合胡萝卜软腐果胶杆菌Pectobacterium carotovorum6个管家基因(pgi、rpoS、mdh、proA、mtlD、icdA)的基因扩增、序列测定和多基因联合系统发育分析,将病原菌鉴定为胡萝卜软腐果胶杆菌P.carotovorum的3个亚种。其中45个菌株为P.carotovorum subsp.odoriferum,频数为83.33%;6个菌株为P.carotovorum subsp.carotovorum,频数为11.11%;3个菌株为P.carotovorum subsp.brasiliensis,频数为5.56%。上述结果显示,北京地区芹菜细菌性软腐病的病原菌为胡萝卜软腐果胶杆菌P.carotovorum的3个亚种,其中以P.carotovorum subsp.odoriferum为优势种。
译  名：
Isolation and identification of the pathogen causing celery bacterial soft rot
作  者：
JIN Zhi-wen;SONG Jia-wei;XIE Xue-wen;CHAI A-li;SHI Yan-xia;LI Bao-ju;The Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;
关键词：
celery;;bacterial soft rot;;Pectobacterium carotovorum;;multilocus sequence analysis;;identification
摘  要：
Celery bacterial soft rot has become severe in Beijing,especially in Shunyi and Tongzhou district.In order to identify the causal agent of the disease,bacterial strains were isolated from the diseased celery stems,and the pathogenicity of the isolates was then confirmed by inoculation of these isolates on celery seedling following Koch's postulation.Fifty-four pathogenic isolates were obtained.Based on morphological observation,physiological,biochemical and Biolog test,as well as multilocus sequence analysis of six concatenated housekeeping gene sequences(pgi、rpoS、mdh、proA、mtlD、icdA),the causal pathogen was identified as Pectobacterium carotovorum.Among these disease-causing isolates,45 strains were identified as P.carotovorum subsp.odoriferum with a frequency of 83.33%,6 strains as P.carotovorum subsp.carotovorum with a frequency of 11.11%,and 3 strains as P.carotovorum subsp.brasiliensis with a frequency of 5.56%.These results indicate that the causing agents of celery bacterial soft rot in Beijing were identified as three subspecies of P.carotovorum,and P.carotovorum subsp.odoriferum was dominant.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46