当前位置: 首页 > 文章 > 纤维素酶和碱性蛋白酶联合提取茶渣中蛋白质的工艺参数研究 安徽农业大学学报 2013,40 (5) 771-777
Position: Home > Articles > Optimizing process parameters for the extract of protein from tea residue by cellulose enzyme and alkaline protease Journal of Anhui Agricultural University 2013,40 (5) 771-777

纤维素酶和碱性蛋白酶联合提取茶渣中蛋白质的工艺参数研究

作  者:
于鹏亮;李立祥;卢昭;张雁飞;薛晨;华再欣;梅玉
单  位:
安徽农业大学理学院;安徽农业大学茶叶生物化学与生物技术教育部;农业部重点实验室;安徽农业大学茶与食品科技学院
关键词:
纤维素酶;碱性蛋白酶;茶渣;蛋白质;响应面设计
摘  要:
研究纤维素酶和碱性蛋白酶联合作用提取绿茶茶渣中蛋白质的工艺。以提取温度、加酶量、pH值、提取时间为参考因素设计实验,响应面设计确定最佳提取条件。结果表明,纤维素酶酶解最佳工艺:温度41℃、加酶量2%、pH值6.4、时间64 min,蛋白质提取率6.58%。碱性蛋白酶酶解工艺:温度60℃、加酶量2%、pH值8.7、时间90 min,蛋白质提取率20.93%。最终双酶联合提取茶叶蛋白质的提取率为32.18%。
译  名:
Optimizing process parameters for the extract of protein from tea residue by cellulose enzyme and alkaline protease
作  者:
YU Peng-liang;LI Li-xiang;LU Zhao;ZHANG Yan-fei;XUE Chen;HUA Zai-xin;MEI Yu;Key Lab of Tea Biochemistry & Biotechnology, Ministry of Education and Agriculture, Anhui Agricultural University;School of Tea and Food Technology, Anhui Agricultural University;School of Science, Anhui Agricultural University;
关键词:
cellulose enzyme;;alkaline protease;;tea residue;;protein;;response surface design
摘  要:
The extraction process of protein from green tea residue by cellulose enzyme and alkaline protease was investigated. Reference factors including the extraction temperature, enzyme dosage, pH value and extraction time were considered to determine the best extraction condition by response surface design. The results showed that the optimum condition for protein extract with cellulose enzyme was 41℃, 2% cellulose enzyme, pH 6.4, and64 min for enzymolysis. The protein extraction rate was 6.58% under the condition. The optimum condition for protein extract with alkaline protease was 60℃, 2% alkaline protease, pH 8.7, and 90 min for enzymolysis. The protein extraction rate was 20.93% under this condition, and the rate with combination of these two enzymes was32.18%.

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