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当前位置: 首页 > 文章 > 新疆雪莲芽分化过程蛋白质·可溶性糖含量及淀粉酶活性变化 安徽农业科学 2009,37 (3) 67-68
Position: Home > Articles > Study on the Change of Protein and Soluble Sugar Contents and Amylase Activity of Xinjiang Saussurea Iinvolucrata during Bud Differentiation Journal of Anhui Agricultural Sciences 2009,37 (3) 67-68

新疆雪莲芽分化过程蛋白质·可溶性糖含量及淀粉酶活性变化

作  者：
徐春明;赵兵
单  位：
中国科学院过程工程研究所生化工程国家重点实验室;北京工商大学化学与环境工程学院北京市植物资源研究开发重点实验室
关键词：
新疆雪莲;愈伤组织;蛋白质;可溶性糖;淀粉酶
摘  要：
[目的]研究新疆雪莲分化过程中的生理生化变化。[方法]将以雪莲无菌苗的叶片为外植体诱导的雪莲胚性愈伤组织置于MS+2 mg/L NAA+0.5 mg/L 6-BA(pH值为5.85~5.90)培养基上进行培养,对培养过程中雪莲细胞可溶性蛋白含量、可溶性糖、淀粉酶等参数进行测定分析。[结果]在新疆雪莲整个培养阶段,胚性愈伤组织中的蛋白质含量高于非胚性愈伤组织,可溶性糖保持稳定,仅在芽分化阶段有一定的上升,α-淀粉酶活性保持稳定。胚性愈伤组织中的β-淀粉酶的活性均高于非胚性愈伤组织,这说明β-淀粉酶与细胞的分化过程密切相关。β-淀粉酶在整个培养过程中呈现双峰曲线。第1个峰出现在培养的开始阶段,第2个峰出现在芽分化时期。[结论]该研究对研究新疆雪莲的高频再生具有重要的指导意义。
译  名：
Study on the Change of Protein and Soluble Sugar Contents and Amylase Activity of Xinjiang Saussurea Iinvolucrata during Bud Differentiation
作  者：
XU Chun-ming et al(Beijing Key Laboratory of Plant Resources Research and Development,College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048)
关键词：
Xinjiang Saussurea involucrata;Callus;Protein;Soluble sugar;Amylase
摘  要：
[Objective] The aim was to study the change of physiology and biochemistry of Xinjiang Saussurea involucrata during differentiation.[Method] The embryogenic callus of S.involucrata induced by the explant that was from the leaves of S.involucrata aseptic seedlings was put on the medium of MS+2 mg/L NAA+0.5 mg/L 6-BA(pH 5.85-5.90)to culture,the parameters of soluble protein content,soluble sugar and amylase etc.of S.involucrata cells during culture process were determined and analyzed.[Result] In the whole culture process of Xinjiang S.involucrata,the protein content of embryonic callus were higher than those of non-embryonic ones,the soluble sugar content kept steady,only had some rise during bud differentiation;α-amylase activity kept steady.The β-amylase activity in embryonic callus was all higher than that of non-embryonic callus,which indicated that β-amylase had a close correlation with differentiation process of cells.β-amylase showed bimodal curve in the whole culture process,the first peak appeared at the beginning stage of culture,and the second peak presented at bud differentiation phase.[Conclusion] The study had important guiding significance in the research of high frequency regeneration of Xinjiang S.involucrata.

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