作 者：
丁耀忠;张杰;刘永生;陈豪泰;曾巧英;马丽娜;马艳平;杨生海;殷宏
单 位：
农业部兽医公共卫生重点开放实验室;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室农业部兽医公共卫生重点开放实验室;农业部畜禽病毒学重点开放实验室;甘肃农业大学
关键词：
牛朊蛋白;二氢叶酸还原酶缺陷型CHO细胞;稳定表达
摘 要：
从T载体上得到的目的基因和dhfr共扩增基因真核表达载体构建了pCI-PrP102。纯化后的重组质粒通过脂质体转染CHO/dhfr-细胞,经MTX加压筛选获得稳定表达的细胞株,间接免疫荧光试验检测到目的蛋白的表达。
译 名：
Stable Expression of Bovine Prion Protein in CHO/dhfr-cells
作 者：
DING Yao-zhong1,2,ZHANG Jie2,LIU Yong-sheng2,CHEN Hao-tai2,ZENG Qiao-ying1,MA Li-na2,MA Yan-ping2,YANG Sheng-hai2,YING Hong2*(1.Gansu Agricultural University,Lanzhou 730070,China;2.Key Laboratory of Veterinary Public Health,Ministry of Agriculture;Key Laboratory of Animal Virology,Ministry of Agriculture;State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)
关键词：
Bovine prion protein;CHO/dhfr-cell;Stable expression
摘 要：
The target genes from T-vector were connected with dhfr co-amplified gene of eukaryotic vector and constructed pCI-PrP102.Purified recombinant plasmids were transferred into CHO/dhfr-cell through liposome,and obtained the stable expression of the cell strains after MTX screening,and indirect immunity fluorescence analysis detected the expression of the goal protein.
