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当前位置: 首页 > 文章 > 赛鸽新城疫病毒南京分离株的生物学特性及其F和HN基因的分析 中国兽医科学 2017,47 (11) 1363-1370
Position: Home > Articles > Biologic characterization and F and HN gene analysis of racing pigeon Newcastle disease virus strain from Nanjing Chinese Veterinary Science 2017,47 (11) 1363-1370

赛鸽新城疫病毒南京分离株的生物学特性及其F和HN基因的分析

作  者：
熊晓妍;孙兴臣;李敏婕;耿文学;万玉萌;孔志龙;陈闻;许家荣
单  位：
南京农业大学动物医学院
关键词：
赛鸽;鸽新城疫;生物学特性;基因型Ⅵ亚型;F基因;HN基因
摘  要：
从南京市某赛鸽公棚发病鸽脑组织中分离到1株鸽新城疫病毒,命名为Pi/NJ/CH/4416/2016(简称ND4416)。各项生物学特性试验结果表明,该分离株为鸽新城疫强毒,对鸽具有高致病性,对鸡的致病性相对较低。经RT-PCR扩增、测序得到分离株的F基因和HN基因序列。F基因分析表明,其裂解位点的氨基酸序列为~(112)R-R-Q-K-R-F117,是典型的强毒株共有序列。F、HN基因遗传进化分析发现,本次分离株的基因型为Class II,VIb亚型,且与目前国内流行株属于同一遗传分支,这些毒株与比利时流行株PPMV-1/Belgium/11-09620/2011(JX901124.1)的亲缘关系相近,推测近年国内流行毒株可能来源于比利时。本次分离毒株与弱毒疫苗株La Sota(AF077761.1)的遗传距离较远。生物信息学分析显示,该鸽源分离株HN蛋白第491~500位的一个线性抗原表位发生了改变,血清学试验也显示本毒株与La Sota疫苗株的抗原性差异明显,在一定程度上说明该鸽源毒株已经发生了抗原性变异。
译  名：
Biologic characterization and F and HN gene analysis of racing pigeon Newcastle disease virus strain from Nanjing
作  者：
XIONG Xiao-yan;SUN Xing-chen;LI Min-jie;GENG Wen-xue;WAN Yu-meng;KONG Zhi-long;CHEN Wen;XU Jia-rong;College of Veterinary Medicine,Nanjing Agricultural University;Nanjing Qianliguang Biotechnology CO.,LTD.;
单  位：
College of Veterinary Medicine,Nanjing Agricultural University%Nanjing Qianliguang Biotechnology CO.,LTD.
关键词：
racing pigeon;;pigeon Newcastle disease;;biological characteristics;;genotype Ⅵb;;F gene;;HN gene
摘  要：
One strain of pigeon Newcastle disease virus(NDV) was isolated from a diseased racing pigeon loft suspected to be infected with NDV in Nanjing. This NDV isolate was named Pi/NJ/CH/4416/2016(ND4416) and purified by using specific pathogen-free(SPF) eggs.Biological tests showed that this isolate showed high pathogenicity to pigeons,but lower to chickens.The resulting RNA samples were converted to c DNAs, followed by PCR amplification and sequencing of F and HN genes.The cleavage site of the fusion protein(F) was112 RRQKRF117,a feature generally associated with virulent NDV strains.Phylogenetic analysis based on F and HN gene sequences showed that this strain belonged to Class Ⅱ,Ⅵb subgenotype.In addition,Pi/NJ/CH/4416/2016 and other strains prevailing in China were more closely related to the PPMV-1/Belgium/11-09620/2011(JX901124.1) strain. It supported that epidemic pigeon NDV isolates in China might originate from Belgium. Pi/NJ/CH/4416/2016 had a far genetic relationship from the classic vaccine strain La Sota(AF077761.1).Bioinformatics analysis revealed that a linear epitopeconsisting of amino acids 491-500 of HN protein of this pigeon strain was different from La Sota.In addition,hemagglutination inhibition test revealed a noticeable antigenic difference between them.

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