中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 甘蓝型油菜小孢子培养技术优化 河南农业科学 2015,44 (5) 53-57
Position: Home > Articles > Optimization of Microspore Culture Techniques of Brassica napus L. Journal of Henan Agricultural Sciences 2015,44 (5) 53-57

甘蓝型油菜小孢子培养技术优化

作  者：
谭少敏;胡靖锋;杨红丽;徐学忠;鄢波;陈倩;施敏;和江明
单  位：
云南省农业科学院;西南林业大学
关键词：
甘蓝型油菜;小孢子培养;胚诱导率;成苗率;加倍效率
摘  要：
为了优化甘蓝型油菜小孢子培养技术,提高胚诱导率及成苗率,以油菜N14009为试验材料进行小孢子培养,研究变温预处理、活性炭浓度、培养基类型和琼脂浓度及秋水仙碱浓度对小孢子培养的影响。结果表明:4℃、32.5℃变温预处理各1 d的处理效果较好,胚诱导率为4.23胚/蕾;活性炭质量浓度为0.5 g/L时,胚诱导率达到4.23胚/蕾,效果最好;MS、B5培养基中琼脂质量浓度为12 g/L时,成苗率均最高,分别达51.16%、46.67%;把再生植株转到含有不同质量浓度秋水碱的MS培养基上培养,质量浓度为80 mg/L时,N14009的加倍率最高,为43.47%,并且成苗率达到76.67%。
译  名：
Optimization of Microspore Culture Techniques of Brassica napus L.
作  者：
TAN Shaomin;HU Jingfeng;YANG Hongli;XU Xuezhong;YAN Bo;CHEN Qian;SHI Min;HE Jiangming;Garden School,Southwest Forestry University;Horticultural Crops Institute,Yunnan Academy of Agricultural Science;
关键词：
Brassica napus L.;;microspore culture;;embryo induction rate;;seedling rate;;doubling rate
摘  要：
In order to optimize microspore culture techniques of Brassica napus L. and improve the embryo induction rate and seedling rate,the N14009 was used as experimental material to culture microspores. During the research,the effects on microspore culture were studied by different temperature pretreatment,concentration of activated carbon,medium type and agar concentration and concentration of colchicine. The results showed that: It got a good result by 4 ℃ and 32. 5 ℃ temperature pretreatments for one day each,and the embryo induction rate was 4. 23 embryos / bud; When the concentration of activated carbon was 0. 5 g / L,the embryo induction rate reached the maximum,4. 23 embryos / bud; In addition,the seedling rate reached 51. 16% and 46. 67% in the MS and B5 media both with 12 g / L of agar,respectively. The regenerated plants were turned to MS that had different concentration of colchicine,the doubling efficiency of N14009 reached 43. 47% and the seedling rate reached 76. 67%,when the concentration of colchicine was 80 mg / L.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46