中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 槲寄生药材亲缘关系的RAPD分析 分子植物育种 2019 (1) 175-180
Position: Home > Articles > RAPD Analysis of the Genetic Relationship of Viscum coloratum(Kom.) Nakai Molecular Plant Breeding 2019 (1) 175-180

槲寄生药材亲缘关系的RAPD分析

作  者：
龙猜;宾文;李艳梅;郭佳鑫;张天园;于治国;赵云丽
单  位：
沈阳药科大学药学院;沈阳药科大学生命科学与生物制药学院
关键词：
槲寄生;RAPD;聚类分析;亲缘关系
摘  要：
本研究以半寄生性药材-槲寄生为研究对象,利用RAPD分子标记技术,以生长环境和寄主植物为要素,旨在探讨这两个因素对槲寄生药材亲缘关系的影响。研究表明:10个RAPD引物在15个槲寄生样本中共扩增出190条带,其中多态性带143条,多态性比率高达75.26%。采用NTSYSpc软件的UPGMA法构建聚类系统树型图,当相似性系数为0.657时,以产地为基础,可以将15个槲寄生样本分为3类。槲寄生样本间的遗传多样性有显著差异:来自河北和黑龙江的槲寄生亲缘关系较近,辽宁地区的槲寄生样本与河北及黑龙江的槲寄生相似性小,亲缘关系相对较远;而来源于不同寄主种属的槲寄生亲缘关系错综复杂,对聚类系谱的划分影响较小。分析结果表明,不同槲寄生药材通过RAPD方法聚类谱系,未能与其寄主种属形成对应关系,但很可能与其生长的地域之间存在一定联系。这为进一步研究槲寄生药材与寄主和产地间的复杂关系提供了更有利的现代研究手段和理论参考。
译  名：
RAPD Analysis of the Genetic Relationship of Viscum coloratum(Kom.) Nakai
作  者：
Long Cai;Bin Wen;Li Yanmei;Guo Jiaxin;Zhang Tianyuan;Yu Zhiguo;Zhao Yunli;School of Medicine,Shenyang Pharmaceutical University;Life Science and Biopharmaceutical School,Shenyang Pharmaceutical University;
单  位：
Long Cai%Bin Wen%Li Yanmei%Guo Jiaxin%Zhang Tianyuan%Yu Zhiguo%Zhao Yunli%School of Medicine,Shenyang Pharmaceutical University%Life Science and Biopharmaceutical School,Shenyang Pharmaceutical University
关键词：
Viscum coloratum;;RAPD;;Cluster analysis;;Genetic relationship
摘  要：
In this study, the se mi-parasitic medicinal material-mistletoe was used as the material, and RAPD molecular marker technology was applied to investigate the effect of growth environment and the host plant on the genetic relationship of mistletoe. The results showed that 10 RAPD primers could amplify 190 bands in 15 mistletoe samples, in which 143 were polymorphic bands, with the polymorphic ratio of 75.26%. The UPGMA method of NTSYSpc software was used to construct the tree map of clustering system. When the similarity coefficient was0.657, 15 mistletoe samples could be divided into 3 categories based on the origin. The genetic diversity of these mistletoe samples had significant differences: the genetic relationship of the mistletoe from Hebei and Heilongjiang was closer, and the mistletoe samples from Liaoning had little similarity to the mistletoe of Hebei and Heilongjiang, and the genetic relationship was relatively distant. While the genetic relationship of different host species was complex, which had little influence on the classification of the pedigree. The analysis results indicated that the different mistletoe herbs clustered by RAPD method, and they did not form the one-to-one relations with their host species, but there was probably a certain relationship with their regions. This research could provide more favorable modern research methods and a theoretical basis for further study on the complex relationship between mistletoe herbs, host and origin.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46