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Position: Home > Articles > Genetic Quality Analysis of Xiphophorus helleri RR-B Strain Using 18 Microsatellite Loci Journal of Anhui Agricultural Sciences 2009,37 (1) 61-63+66

利用18个微卫星座位对剑尾鱼RR-B系遗传质量的分析

作  者:
刘宇飞;白俊杰;全迎春;叶星;黄志斌
单  位:
中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室
关键词:
剑尾鱼;近交系;微卫星;遗传监测
摘  要:
[目的]为进一步了解剑尾鱼RR-B系的遗传质量,探讨微卫星标记技术在水生实验动物遗传监测中应用的可行性。[方法]选择18个在野生剑尾鱼群体中有多态性的微卫星座位,通过PCR扩增对剑尾鱼RR-B系(红眼红体)遗传质量进行分析。[结果]18个座位在RR-B系30尾个体中均为单态,且都是纯合子,基因纯合率达100%。[结论]18个微卫星座位可作为剑尾鱼RR-B系遗传监测的分子标记;剑尾鱼RR-B系经20多代的近交培育已具有很高的遗传均一性。该研究为水生实验动物分子监测标准的制定提供了基础数据。
译  名:
Genetic Quality Analysis of Xiphophorus helleri RR-B Strain Using 18 Microsatellite Loci
作  者:
LIU Yu-fei et al (Key Lab of Tropical and Subtropical Fish Breeding & Cultivation,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510380)
关键词:
Xiphophorus helleri;Inbred strain;Microsatellite;Genetic monitoring
摘  要:
[Objective] The study aimed to further understand the genetic quality of Xiphophorus helleri RR-B strain and to estimate the feasibility of microsatellite markers in genetic monitoring of aquatic laboratory animals.[Method] Eighteen microsatellite loci which had polymorphism in wild X.helleri were used for genetic quality analysis of RR-B strain(red eyes and red body) by PCR.[Result] All these 18 microsatellite loci displayed single allelic gene band in 30 individuals of X.helleri RR-B strain and the average homozygote rate reached 100%.[Conclusion] These loci could be used as markers in genetic monitoring of X.helleri RR-B strain,which had obtained fairly high genetic homozygosity by inbreeding for more than 20 generations.This study provided basic data for establishing the molecular genetic monitor standard of aquatic laboratory animals.

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