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当前位置: 首页 > 文章 > 具有光肩星天牛内切聚葡糖酶结合活性短肽的筛选 林业科学研究 2006,19 (3) 267-271
Position: Home > Articles > Selecting and Identification of Binding Peptides of the Endoglucanases from Anoplophora glabripennis Forest Research 2006,19 (3) 267-271

具有光肩星天牛内切聚葡糖酶结合活性短肽的筛选

作  者：
陈敏;卢孟柱;王敏杰;张志毅
单  位：
北京林业大学教育部林木花卉遗传育种重点实验室;北京林业大学省部共建森林培育与保护教育部重点实验室;中国林业科学研究院林业研究所国家林业局林木培育重点实验室
关键词：
光肩星天牛;内切葡聚糖酶;随机多肽噬菌体展示库;短肽;亲和性
摘  要：
内切葡聚糖酶(endoglucanases)是光肩星天牛幼虫肠道的主要纤维素消化酶。本研究以光肩星天牛内切葡聚糖酶的同工酶AgEG2为靶分子,从随机多肽噬菌体展示库中筛选与AgEG2有亲和活性的短肽,通过3轮筛选,短肽序列TPHRSPL出现频率为33.7%,而且展示该短肽的噬菌体均对AgEG2有很高的结合能力。进一步合成短肽TPHRSPL,并对肠道纤维素酶提取液进行了W estern分析,结果表明该短肽能特异结合内切葡聚糖酶的同工酶AgEG1和AgEG2,而与粗酶液中其它蛋白组分均无结合特性。表明筛选获得的短肽TPHRSPL对光肩星天牛内切葡聚糖酶具有特异结合亲和性。该短肽为研究光肩星天牛纤维素酶的特性及开发天牛的生物防治制剂奠定了基础。
译  名：
Selecting and Identification of Binding Peptides of the Endoglucanases from Anoplophora glabripennis
作  者：
CHEN Min~(1,2),LU Meng-zhu~2,WANG Min-jie~2,ZHANG Zhiyi~3(1.Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Beijing 100083,China;2.Research Institute of Forestry,CAF,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,China;3.Key Laboratory of Genetics and Breeding in Trees and Ornamental Plant,Ministry of Education,Beijing Forestry University,Beijing 100083,China)
关键词：
Anoplophora glabripennis;endoglucanases;random peptides phage display library;binding peptides;affinity
摘  要：
Endoglucanases are the main cellulolytic enzymes in the gut of Anoplophora glabripennis.In this study,random peptide phage display technology was employed to screen peptides that bound the AgEG2,a member of endoglucanase isozymes.Phage clones displaying peptide TPHRSPL accounted for 33.7% of the selected phage population after three rounds of screening,and showed higher phage recovery than the other clones in the binding assay.Peptide TPHRSPL was chemically synthesized and tested for its binding activity to AgEG2.The synthetic peptide exhibited high binding specificity for AgEG1 and AgEG2.This indicated that peptide TPHRSPL had the affinity to the endoglucanase of A.glabripenni,which could be used to study the biological role of the enzyme in the gut,and had the potential to be developed into biological control agents of A.glabripenni.

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