关键词：
血管紧张素转化酶2(ACE2);基因敲除鼠;造模给药;动脉粥样硬化;血脂;肝脏病变
摘 要：
为了研究血管紧张素转化酶2(ACE2)在动脉粥样硬化模型的小鼠中发挥的作用,试验采用8~10周龄APOE基因敲除小鼠,分为普通饮食对照组、高脂饮食模型组、阳性药物对照组(卡托普利给药组)及ACE2激动剂组[血管紧张素转化酶2(ACE2)激动剂重氮氨苯脒乙酰甘氨酸盐(DIZE)给药组],皮下注射给药12周后处死,观察小鼠的肝脏病理学特征,并检测相应血清指标。结果表明:高脂饮食模型组三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)比普通饮食对照组显著升高,高密度脂蛋白(HDL)降低,且肝组织脂肪变;而给予卡托普利和DIZE的药物组后都相应降低了TG、TC、LDL含量,提高了HDL含量,并且能够很大程度地减轻肝组织的脂肪病变程度。说明动脉粥样硬化发生的进程中,血脂升高,肝脏也发生了相应病变。而ACE2激动剂DIZE和血管紧张素转化酶抑制剂卡托普利一样,可以降低血脂,改善局部的肝脏病变。
译 名：
Study on the role of angiotensin converting enzyme 2(ACE2) agonist in atherosclerotic liver
作 者：
LIN Jie;SUN Huirong;SHAO Juan;ZHANG Youzhi;WU Jiliang;School of Basic Medical Sciences,Wuhan University;Institute of Medicine,Hubei University of Science and Technology;
关键词：
angiotensin converting enzyme 2(ACE2);;gene-knockout mouse;;modeling and administration;;atherosclerosis;;blood lipid;;he patic lesion
摘 要：
To investigate the role of angiotensin converting enzyme 2( ACE2) in a mouse model of atherosclerosis,the APOE gene- knockout mice from eight to ten weeks of age were divided into four groups,including normal diet group as a control,high- fat diet model group,positive- drug control group( captopril administration group),and angiotensin converting enzyme 2( ACE2) agonist group( ACE 2 agonist diminazene aceturate( DIZE) administration group). The mice were sacrificed after administration of subcutaneous injection for 12 weeks,and then the liver pathological characteristics were observed,while the serum indexes in mice were accordingly detected. The results showed that the levels of triacylglycerol( TG),total cholesterol( TC),low- density lipoprotein( LDL) in the high- fat diet model group were significantly higher than those in the control group,while the level of high- density lipoprotein( HDL) was decreased,appearing liver steatosis. However,the levels of TG,TC,and LDL in the drug groups administrated with captopril and DIZE were all decreased,and the HDL level was increased,which could greatly reduce pathological changes of hepatic tissues. The results indicate that blood lipids are increased and the corresponding pathological changes occurs in liver in the process of atherosclerosis occurring,while the ACE2 agonist DIZE and ACE inhibitor captopril can reduce blood lipids and improve the partial pathological changes in liver.
