中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 三氯生对雌性斑马鱼肝胰脏凋亡相关基因表达的影响 广东海洋大学学报 2020 (1) 15-18
Position: Home > Articles > Effect of Triclosan on Expression of Apoptosis-related Genes in Female Zebrafish Hepatopancreas Journal of Guangdong Ocean University 2020 (1) 15-18

三氯生对雌性斑马鱼肝胰脏凋亡相关基因表达的影响

作  者：
刘旭昊;祖恩普;王凡
单  位：
洛阳师范学院
关键词：
三氯生;斑马鱼;雌性斑马鱼;肝胰脏损伤;凋亡基因;实时荧光定量PCR
摘  要：
【目的】研究三氯生(TCS)对雌性硬骨鱼类肝胰脏损伤的相关分子机制。【方法】采用半静态水体接触染毒法,将雌性斑马鱼(Danio rerio)暴露于0、0.017、0.034、0.068 mg/L的TCS溶液42 d,采用普通PCR和实时荧光定量PCR技术测定雌性斑马鱼肝胰脏凋亡相关基因(Bcl-2、p53、MDM2、Bax)的表达情况。【结果】0.017～0.068 mg/L组的雌性斑马鱼肝胰脏Bcl-2、MDM2和Bax基因的表达极显著下调(P <0.01),p53基因的表达极显著上调(P <0.01)。【结论】0.017～0.068 mg/L的TCS显著影响雌性斑马鱼肝胰脏凋亡相关基因的表达,促进雌性斑马鱼肝胰脏细胞凋亡的发生。
译  名：
Effect of Triclosan on Expression of Apoptosis-related Genes in Female Zebrafish Hepatopancreas
作  者：
LIU Xu-hao;ZU En-pu;WANG Fan;Life Science Department, Luoyang Normal University;
关键词：
triclosan;;Danio rerio;;female zebrafish;;hepatopancreas injury;;apoptosis gene;;real-time fluorescence quantitative PCR
摘  要：
【Objective】To study the molecular mechanism of triclosan(TCS) on hepatopancreas injury in the female bony fish.【Method】Zebrafish(Danio rerio) were exposed to TCS solution of 0, 0.017, 0.034, and 0.068 mg/L respectively by semi-static water exposure for 42 days, and the expressions of apoptosis-related gene(Bcl-2, p53, MDM2, and Bax) in the hepatopancreas were detected by PCR and real-time quantitative PCR. 【Results】Compared with the control group, the expressions of Bcl-2, MDM2 and Bax gene in the TCS treated groups were significantly down-regulated(P< 0.01), and P53 gene was significantly up-regulated in the TCS treated group(P < 0.01). 【Conclusion】TCS has a significant effect on the expression of apoptosis-related genes in the hepatopancreas of zebrafish and may accelerate apoptosis in the hepatopancreas of female zebrafish.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46