中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 空肠弯曲菌CRISPR-Cas系统生物信息学分析 中国预防兽医学报 2018 (10) 960-964
Position: Home > Articles > Bioinformatic analysis of CRISPR-Cas system in Campylobacter jejuni Chinese Journal of Preventive Veterinary Medicine 2018 (10) 960-964

空肠弯曲菌CRISPR-Cas系统生物信息学分析

作  者：
张博;姚学萍;曹随忠;王印;杨泽晓
单  位：
四川农业大学动物医学院;动物疫病与人类健康四川省重点实验室
关键词：
空肠弯曲菌;CRISPR-Cas系统;重复序列;间隔序列;生物信息学
摘  要：
为分析GenBank中登录的国内外空肠弯曲菌及其亚种中所含CRISPR-Cas系统的结构特征及同源关系,本研究利用生物信息学方法预测GenBank中已登录的空肠弯曲菌所含的CRISPR-Cas系统的基因序列,对其进行比对、保守性分析、RNA二级结构预测、重复序列的GC含量分析及其遗传进化分析。结果显示,空肠弯曲菌及其亚种中所含CRISPR-Cas系统的重复序列高度保守、GC含量较低(28.57%以下),差异性主要呈现于两端游离部分;CRISPR中的间隔序列差异性较大致使CRISPR具有多态性;Cas蛋白序列同源性较高(96.8%以上),遗传动态变化较低,与基因水平转移产生的趋同进化有潜在相关性。空肠弯曲菌CRISPR-Cas系统中的间隔序列具有多态性与该菌的遗传进化差异有关,重复序列及cas基因保守性较强,与其共同进化有潜在相关性。通过对不同空肠弯曲菌CRISPR-Cas系统生物信息学分析,为进一步研究该菌CRISPR-Cas系统结构特征、基因进化关系奠定了基础。
译  名：
Bioinformatic analysis of CRISPR-Cas system in Campylobacter jejuni
作  者：
ZHANG Bo;YAO Xue-ping;CAO Sui-zhong;WANG Yin;YANG Ze-xiao;College of Veterinary Medicine, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;
关键词：
Campylobacter jejuni;;CRISPR-Cas system;;repeat sequence;;spacer sequence;;bioinformatics
摘  要：
To analyze the structural features and homology relations of the CRISPR-Cas system in Campylobacter jejuni available in GenBank, the gene sequence of CRISPR-Cas system in C.jejuni from GenBank was predicted by the bioinformatics methods. The comparison, conservative analysis, RNA secondary structure prediction and GC content analysis of the repeat sequences of CRISPR-Cas system in C.jejuni were performed, and the genetic evolution trees of repeat sequences were constructed. The results showed that the conservation of repeat sequences of CRISPR-Cas system in C.jejuni was high and GC content was below 28.57%. The difference appeared in both end of repeat sequence and the main difference of spacer sequence resulted in the polymorphism of CRISPR. The homology of Cas protein sequence was over 96.8%, and the genetic dynamic change was weak, which potentially related to the convergent evolution of gene level shifts. The polymorphism of spacer sequence of CRISPR-Cas system in C.jejuni was related to the genetic evolutionary difference, and the conservation of repeat sequence and cas genes was high, and there was a potential correlation with the coevolution of repeat sequence and cas genes. Analyzing the biological information of CRISPR-Cassystem in different C.jejuni could lay the foundation for further study on the structural characteristics and evolution relationship of CRISPR-Cas system.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46