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提高PCR产物的几种有效方法

作  者:
李爱丽;姜涛;马峙英;贾继增
单  位:
中国农业科学院作物品种资源研究所农业部作物种质资源与生物技术重点开放实验室;河北农业大学农学院
关键词:
聚合酶链式反应;PCR引物;GC富集区;梯度PCR
摘  要:
结合作者的工作实际 ,着重介绍了有效提高PCR产物的几种方法 :如怎样高效快速地设计PCR引物 ,怎样尽快确定PCR反应的最适退火温度 ,如何提高GC富集区的扩增效率等 ,为分子生物学工作者提供一些可以借鉴的方法及经验。
译  名:
Several Methodologies on Improving the PCR Products
作  者:
Li Aili 1, 2 Jiang Tao 1 Ma Zhiying 2 Jia Jizeng 1 ( 1 Key Laboratory of Crop Germplasm Resources and Biotechnology Ministry of Agriculture Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences,Beijing 100081; 2 Agricultural University of HeBei, Baoding 071001)
关键词:
Polymerase chain reaction PCR primer GC rich region Gradient PCR
摘  要:
Combining the author's working experiences, we introduce here several methodologies on improving the PCR products, such as the disciplines which we should abide by when we design PCR primers; how to determine the appropriate annealing temperature of the PCR reaction; how to improve the amplifying efficiency of the GC rich region. We hope to provide some methodologies for those who do research in microbiology.

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