当前位置: 首页 > 文章 > 降解纤维素嗜热菌的分离及纤维素酶性质分析 福建农林大学学报(自然科学版) 2010,39 (1) 69-74
Position: Home > Articles > Isolation of cellulose-degradation thermophilic bacteria and character of cellulase Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2010,39 (1) 69-74

降解纤维素嗜热菌的分离及纤维素酶性质分析

作  者:
陈路劼;刘斌;林白雪;何柳;张宁;吴祖建;谢联辉
单  位:
福建农林大学植物病毒研究所
关键词:
嗜热菌;降解纤维素;耐高温纤维素酶
摘  要:
从福州永泰温泉分离到1株降解纤维素嗜热菌,通过形态特征和16S rDNA序列鉴定,将其命名为地芽孢杆菌(Geobacillussp.)TC-S8.采用3,5-二硝基水杨酸显色法(DNS法)对菌株TC-S8产胞外纤维素酶的发酵条件及酶性质进行研究.在培养基的初始pH为6.5,温度为60℃,装液量20%,接种量2%,摇床转速为120 r.min-1的条件下培养36 h,菌株TC-S8产酶量达到最大(229 mU.mL-1).其分泌的纤维素酶最适作用温度为60-70℃,最适pH为7.0.纤维素酶降解产物主要为葡萄糖,并有少量纤维二糖.
译  名:
Isolation of cellulose-degradation thermophilic bacteria and character of cellulase
作  者:
CHEN Lu-jie1,LIU Bin1,2,LIN Bai-xue1,HE Liu1,ZHANG Ning1,WU Zu-jian1,XIE Lian-hui1(1.Institute of Plant Virology;2.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
关键词:
thermophilic bacteria;cellulose degradation;thermostable cellulase
摘  要:
A cellulose-degradation thermophilic bacteria was isolated from hotsprings of Yongtai County in Fuzhou,which was named as Geobacillus sp.TC-S8 by identification of morphological characteristics and 16S rDNA sequence.Ferment condition and character of the extracellular cellulase of Geobacillus sp.TC-S8 were analyzed with 3,5-dinitrosalicylic acid.The enzyme yield of strain TC-S8 reached its peak(229 mU·mL-1) after culturing 36 h at the conditions of initial pH of culture medium was 6.5,temperature was 60 ℃,broth content was 20%,inoculum was 2% and rotation speed was 120 r·min-1.The optimum temperature of the cellulase excreted by strain TC-S8 was 60-70 ℃ and the optimum pH was 7.0.The degradation products were mainly glucose and a little cellobiose.

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