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当前位置: 首页 > 文章 > 香樟根际促生菌的筛选与促生特性研究 北方园艺 2019 (4) 59-64
Position: Home > Articles > Isolation and Promoting Characteristics of the Rhizospheric Bacteria of Cinnamomum camphora Northern Horticulture 2019 (4) 59-64

香樟根际促生菌的筛选与促生特性研究

作  者：
林英;司春灿;黄莉萍;黄云祥;廖瑜璐
单  位：
景德镇学院生物与化学工程系;湖南大学隆平分院;南昌大学生命科学研究院
关键词：
香樟;根际促生菌;筛选;促生特性
摘  要：
以香樟根际土壤为试材,采用PVK平板检测法、亚历山大平板检测法、CAS平板检测法、JNFb培养基检测法、Salkowski比色法,分别研究了从香樟根际土中随机挑取的22株细菌的解磷、解钾、产铁载体、固氮、产吲哚-3-乙酸(IAA)能力,采用16S rDNA对产铁载体和产IAA能力较强的菌株进行了分子鉴定,以期为探讨香樟速生、耐贫瘠等机制,同时也为香樟及其它植物研制微生物菌肥提供一定的参考依据。结果表明:挑取的22株细菌中,有7株具有解磷能力,8株具有固氮能力,11株能产铁载体,16株能产IAA。对初步分离的具有产铁载体和产IAA的菌株进行进一步的定量测定发现,菌株XZ3和XZ22产生的铁载体活性单位明显高于其它菌株,分别为78.4%和74.6%。XZ11和XZ13产IAA的能力最强分别为46.82、46.19μg·mL~(-1)。经16S rDNA分子鉴定,XZ3和XZ22分别隶属短小芽孢杆菌(Lysinibacillus sp.)和芽孢杆菌(Bacillus sp.),XZ11和XZ13分别隶属假单胞菌(Pseudomonas sp.)和短小芽孢杆菌(Lysinibacillus sp.)。
译  名：
Isolation and Promoting Characteristics of the Rhizospheric Bacteria of Cinnamomum camphora
作  者：
LIN Ying;SI Chuncan;HUANG Liping;HUANG Yunxiang;LIAO Yulu;Department of Biological and Chemical Engineering,Jingdezheng University;College of Longping,Hunan University;Institute of Life Sciences,Nanchang University;
单  位：
LIN Ying%SI Chuncan%HUANG Liping%HUANG Yunxiang%LIAO Yulu%Department of Biological and Chemical Engineering,Jingdezheng University%College of Longping,Hunan University%Institute of Life Sciences,Nanchang University
关键词：
Cinnamomum camphora;;rhizospheric bacteria;;screen;;promoting characteristics
摘  要：
In order to provide some reference value for studying the mechanism of Cinnamomum camphora′s fast growth and good adaptability to infertile soil,meanwhile for development microbial fertilizer for C.camphora and other plants.Taking the rhizosphere soil of C.camphora as test materials,22 strains were random took from C.camphora rhizosphere soil,using PVK solid soluble phosphorus method,Alexander plate assay method,CAS plate assay method,JNFb medium assay method,and Salkowski colorimetric method to respectively to test their phosphate solubilisation activity,potassium solubilisation activity,secreting iron ability,nitrogen fixation ability and producing IAA ability.The strains performed stronger secreting iron ability and producing IAA ability were selected for 16 S rDNA molecular identification.The results showed that of the 22 strains isolated,7 strains had phosphate solubilizing ability,8 strains were capable of nitrogen fixation,11 strains were capable of producing iron,and 16 strains could produce IAA.Further quantitative determination of the initial isolated strains with siderophore and IAA,the result was found that the siderphore units of XZ3(78.4%) and XZ22(74.6%) were significantly higher than those of other strains.XZ11 and XZ13 were the most capable of producing IAA with 46.82 μg·mL~(-1) and 46.19 μg·mL~(-1) respectively.XZ3 and XZ22 belonged to Lysinibacillus sp.and Bacillus sp.,XZ11 and XZ13 belonged to Pseudomonas sp.and Lysinibacillus sp.respectively by the 16 Sr DNA.

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