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当前位置: 首页 > 文章 > 不同铜源化合物在水溶液或细胞培养液中铜离子价态研究 中国畜牧杂志 2018 (9) 100-104
Position: Home > Articles > Study on the Copper Ion Valence State of Different Copper Source Compounds in Aqueous Solution or Cell Culture Solution Chinese Journal of Animal Science 2018 (9) 100-104

不同铜源化合物在水溶液或细胞培养液中铜离子价态研究

作  者：
张艳玲;朱风华;陈甫;李玲;巩青军;朱连勤
单  位：
青岛农业大学动物医学院;青岛农业大学生命科学学院;青岛农业大学动物科技学院
关键词：
纳米氧化铜;络合剂;细胞培养液;一价铜离子;二价铜离子
摘  要：
本试验旨在研究硫酸铜、微米氧化铜和纳米氧化铜在水溶液或细胞培养液中解离出的铜离子价态,为进一步研究纳米氧化铜在体内消化道的吸收转运方式提供理论基础。配制铜水平为8 mg/L的硫酸铜、微米氧化铜、纳米氧化铜的超纯水溶液为超纯水对照组,在超纯水对照组基础上分别添加2倍铜摩尔含量的一价铜离子络合剂(BCS)或0.5倍铜摩尔含量的二价铜离子络合剂(EDTO);不同铜源化合物的细胞培养液配制及BCS和EDTO添加方式同超纯水溶液试验,分别于15、30、60、120、240、480 min检测超滤液中铜含量。结果表明:在纳米氧化铜水溶液或细胞培养液中分别添加EDTO、BCS,超滤液中铜含量均分别小于对照组(P<0.05),在微米氧化铜、硫酸铜水溶液中或细胞培养液中分别添加EDTO,超滤液中铜含量均分别小于对照组(P<0.05);在微米氧化铜、硫酸铜水溶液中或细胞培养液中分别添加BCS,超滤液中铜含量与对照组差异不显著(P>0.05)。在水溶液中和细胞培养液中,超滤液中最终解离出的铜离子含量均为硫酸铜组>纳米氧化铜组>微米氧化铜组(P<0.05)。由此可见,在水溶液或细胞培养液中,硫酸铜、微米氧化铜解离产生Cu2+、纳米氧化铜解离产生Cu2+和Cu+。
译  名：
Study on the Copper Ion Valence State of Different Copper Source Compounds in Aqueous Solution or Cell Culture Solution
作  者：
ZHANG Yan-ling;ZHU Feng-hua;CHEN Fu;LI Ling;GONG Qing-jun;ZHU Lian-qin;College of Veterinary Medicine, Qingdao Agricultural University;College of Animal Science and Technology, Qingdao Agricultural University;College of Life Science,Qingdao Agricultural University;
关键词：
Nano-copper oxide;;Complexing agent;;Cell culture medium;;Monovalent copper ion;;Bivalent copper ion
摘  要：
This experiment aimed to study the dissociation of copper ion valence in copper sulfate, micron-copper oxide and nano-copper oxide in aqueous solution or simulated intestinal cell culture solution, and it provided a theoretical basis for further study on the absorption and transportation of nano-copper oxide in the digestive tract of the body. The ultra-pure aqueous solution of copper sulfate, micro-copper oxide, and nano-copper oxide with a copper level of 8 mg/L was prepared as a control group of ultra-pure aqueous solution, and the monovalent copper ion Complexing agents(BCS) with the twice copper molar content or 0.5 times copper molar content of divalent copper ion complexing agents(EDTO)was added on the basis of the ultra-pure aqueous control group. preparation of different cell cultures of copper source compounds and addition of BCS and EDTO in the same manner as the ultrapure aqueous solution. The copper content in the ultrafiltrate was detected at 15, 30, 60, 120, 240 and 480 min, respectively. The results showed that EDTO and BCS were added to the nano-copper oxide aqueous solution or cell culture solution, and the content of ultrafiltration copper was significantly less than that of the control group, respectively(P<0.05). EDTO was added to micron-copper oxide, copper sulfate aqueous solution or cell culture solution, and the content of copper in ultrafiltration was significantly less than that of the control group, respectively(P<0.05). BCS was added to micron-copper oxide, copper sulfate aqueous solution or cell culture solution, and there was no significant difference in the content of ultrafiltration copper from the control group(P>0.05). In addition, in the aqueous solution and cell culture solution, the final dissociated copper ion content was significantly higher in the copper sulfate group than in the nano-copper oxide group and also significantly higher than the micron-copper oxide group(P<0.05). Thus, the dissociation of copper sulfate and micron-copper oxide produced Cu~(2+) and the dissociation of nano-copper oxide produce Cu~(2+) and Cu~+ in the aqueous solution or cell culture solution.

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