Position: Home > Articles > Analysis on rDNA ITS Sequence of the Green-tides in the Coast Areas of Jiangsu Province
Biotechnology Bulletin
2011,0
(3)
135-140
江苏海域几种绿潮藻类rDNA ITS序列分析
作 者：
陈淑吟;胡传明;陆勤勤;孙中响;许广平;丁亚平
单 位：
江苏省海洋水产研究所
关键词：
绿潮藻类;浒苔;石莼;盘苔;ITS序列;分类鉴定
摘 要：
于2009年5-7月定点采集了江苏海域绿潮藻类,测定、分析了这些藻类核糖体rDNA ITS序列,并进行分类鉴定。研究结果显示,ITS+5.8S序列片段长度为552-578 bp,其中ITS1序列部分长度为179-182 bp,5.8S序列全长为155-158 bp,ITS2序列全长为180-196 bp,序列的平均GC含量(contents)为61.6%-63.3%,不同ITS序列存在不同的插入/缺失位点。扩增得到27个序列中有7个不同序列,依据NCBI数据库相似性查找、系统进化关系及遗传距离等分析结果,确定有4个种类:浒苔(Ulva prolifeya),缘管浒苔(U.linza),石莼属(Ulva sp.)1种及盘苔属(Blidingia sp.)1种。分析表明,ITS序列可作为石莼科种类鉴定的标记。
译 名：
Analysis on rDNA ITS Sequence of the Green-tides in the Coast Areas of Jiangsu Province
作 者：
Chen Shuyin1 Hu Chuanming1 Lu Qinqin1 Sun Zhongxiang1,2 Xu Guangping1 Ding Yaping1(1Marine Fisheries Research Institute of Jiangsu Province,Nantong 226007; 2School of Preclinical Medicine and Biological Science,Soochow University Medical Department,Suzhou 215123)
关键词：
Green-tides Ulva Enterornorpha Blidingia ITS region Phylogenetic relationship
摘 要：
In order to investigate the green-tides composition and dominant algae in the coastal waters of Jiangsu province,from May to July in 2009,algae samples were collected from the costal regions(119°32′-122°00′E,32°25′-36°49′N),and sequenced the rDNA internal transcribed spacer(ITS)fragments.The results showed that,the length of ITS + 5.8S rDNA sequences were 552-578 bp,partial sequence of ITS1 was 179-182 bp,complete sequences of 5.8S and ITS2 were 155-158 bp and 180-196 bp,respectively,the average GC contents of the sequence was 61.6%-63.3%.27 samples were sequenced and 7 sequences were found different,which were identified as 4 species,including:Ulva prolifeya,U.linza,U.sp.and Blidingia sp..Sequences were compared and used to construct the phylogenetic trees and showed that the ITS sequence would be a useful tool for identification in Ulvaceae family algae.
相似文章
-
从藻类生理生态学角度解析绿潮爆发的内在机制 [马汶菲, 李景玉] 应用生态学报 2022,33 (5) 1420-1428
