Vitamin C Requirement of Juvenile Centropristis striata

ZHAO Tingting;CHEN Chao;SHAO Yanxiang;ZHANG Qingwen;LIU Ling;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science;College of Fishers and Life Science,Shanghai Ocean University;

ZHAO Tingting%CHEN Chao%SHAO Yanxiang%ZHANG Qingwen%LIU Ling%Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science%College of Fishers and Life Science,Shanghai Ocean University

Centropristis striata;;vitamin C;;growth;;antioxidant;;vitamin C accumulation

This experiment was conducted to investigate the vitamin C requirement of juvenile Centropristis striata. A single factor test design was adopted in the experiment,a total of 630 juvenile Centropristis striata with average body weight of( 88.34±24.10) g were selected and randomly divided into 7 groups with 3 replicates per group and 30 fishes per replicate. Fishes in the 7 groups were fed the isonitrogenous and isocaloric diets which vitamin C contents( measured values) were 10. 97( control),74. 03,148. 33,213. 64,335. 33,618.88,910. 98 mg/kg,respectively. The effects of dietary vitamin C on growth performance,serum biochemical indices,related enzyme activities,tissue vitamin C concentrations and muscle composition of juvenile Centropristis striata were detected. The experiment lasted for 8 weeks. The results showed as follows: 1) the weight gain rate and special gain rate in 74. 03 mg/kg group were significantly higher than those in 10. 97,618.88 and 910.98 mg/kg groups( P<0.05),the feed conversion ratio in 74.03 mg/kg group was significantly lower than that in 10. 97 and 910. 98 mg/kg groups( P < 0. 05),the protein efficiency ratio in 10. 97 and910.98 mg/kg groups was significantly lower than that in 74. 03 and 618. 88 mg/kg groups( P < 0. 05),the condition factor in 335.33 mg/kg group was significantly lower than that in 74.03,148.33 and 213.64 mg/kg groups( P<0.05). 2) The serum total protein content in 10.97 and 74.03 mg/kg groups was significantly low-er than that in other groups( P<0.05),the serum triglyceride content in 618.88 and 910.98 mg/kg groups was significantly lower than that in other groups( P < 0. 05),the serum lysozyme activity in 10. 97 mg/kg group was significantly lower than that in 213.64,335.33,618.88 and 910.98 mg/kg groups( P<0.05). 3) The serum superoxide dismutase( SOD) activity in 335.33 mg/kg group was significantly higher than that in 10.97,74.03 and 910.98 mg/kg groups( P<0.05),the serum total antioxidant capacity( T-AOC) in 335.33 mg/kg group was significantly higher than that in 10. 97,618. 88 and 910. 98 mg/kg groups( P < 0. 05),the serum malondialdehyde( MDA) content in 335.33 mg/kg group was significantly lower than that in 910.98 mg/kg group( P<0.05). 4) The activities of SOD and catalase( CAT) in liver in 10.97 mg/kg group were significantly lower than those in other groups( P < 0. 05), the liver MDA content in 148. 33,335. 33 and910.98 mg/kg was significantly lower than that in 10.97 mg/kg group( P<0.05). 5) The muscle SOD activity and T-AOC in 74.03 mg/kg group were significantly higher than those in other groups( P<0.05). 6) The serum glutamic oxalacetic transaminase( GOT) activity in 213.64,335.33,618.88 and 910.98 mg/kg groups was significantly lower than that in 10. 97 mg/kg group( P < 0. 05),the liver GOT activity in 10. 97 mg/kg group was significantly lower than that in other groups( P < 0. 05). The serum glutamic-pyruvic transaminase(GPT) activity in 10. 97 and 74. 03 mg/kg groups was significantly higher than that in other groups( P <0.05). 7) The serum vitamin C accumulation in 910.98 mg/kg group was significantly higher than that in other groups( P<0.05),the liver vitamin C accumulation in 910. 98 mg/kg group was significantly higher than that in 10.97,74. 03,148. 33 and 213. 64 mg/kg groups( P < 0. 05),the muscle vitamin C accumulation in618.88 mg/kg group was significantly higher than that in 10. 97,74. 03,148. 33 and 213. 64 mg/kg groups( P<0.05). 8) The muscle moisture content in 74.03 mg/kg group was significantly lower than that in 10.97,148.33 and 213.64 groups( P<0.05),the muscle collagen content in 910. 98 mg/kg group was significantly higher than that in other groups( P<0.05). In conclusion,set the special gain rate,liver vitamin C accumulation and serum lysozyme activity as evaluation indexes,the broken-line regression analysis results indicate that dietary vitamin C requirements of juvenile Centropristis striata are 66.66,309.93 and 345.11 mg/kg,respectively.