Position: Home > Articles > Effects of Vitamin D_3 on the Relative Expression Levels of Interleukin 2 and Interleukin 18 Genes in Tissues of Silky Fowls
Chinese Journal of Animal Nutrition
2014,26
(1)
197-202
维生素D_3对丝毛乌鸡组织白细胞介素2和白细胞介素18基因相对表达量的影响
作 者：
李思明;唐艳强;谷德平;肖海红;韦启鹏;欧阳玲花
单 位：
江西省农业科学院畜牧兽医研究所
关键词：
丝毛乌鸡;维生素D 3;白细胞介素
摘 要：
本试验旨在研究维生素D3对丝毛乌鸡组织白细胞介素2(IL-2)和白细胞介素18(IL-18)基因相对表达量的影响。试验选用120只丝毛乌鸡,随机分为5个处理,每个处理4个重复,每个重复6只鸡。对照处理饲喂基础饲粮,各试验处理分别饲喂在基础饲粮中添加800、1 600、3 200和6 400 IU/kg维生素D3的试验饲粮。常规笼养6周。采集胸腺、十二指肠、空肠、回肠、盲肠和法氏囊,检测IL-2和IL-18基因相对表达量。结果表明:6 400 IU/kg的维生素D3上调了IL-2基因在十二指肠、法氏囊和盲肠组织中的相对表达量,而未见其在胸腺、空肠和回肠组织中的相对表达量上调;6 400 IU/kg的维生素D3上调了IL-18基因在丝毛乌鸡胸腺、回肠、盲肠和法氏囊组织中的相对表达量。结果提示,饲粮维生素D3添加水平与丝毛乌鸡体内IL-2和IL-18基因相对表达量相关,但作用存在组织差异。
译 名：
Effects of Vitamin D_3 on the Relative Expression Levels of Interleukin 2 and Interleukin 18 Genes in Tissues of Silky Fowls
作 者：
LI Siming;TANG Yanqiang;GU Deping;XIAO Haihong;WEI Qipeng;OUYANG Linghua;Institute of Animal Husbandry and Veterinary Jiangxi Academy of Agricultural Sciences;
关键词：
Silky fowl;;vitamin D 3;;interleukin
摘 要：
The aim of this experiment was to research the effects of vitamin D 3 on the relative expression levels of interleukin 2( IL-2) and interleukin 18( IL-18) genes in tissues of Silky fowls. One hundred and twenty Silky fowls were randomly allotted to 5 treatments with 4 replicates in each treatment and 6 fowls per replicate. Fowls in control treatment were fed a basal diet,while those in experimental treatments were fed the basal diet supplemented with 800,1 600,3 200 and 6 400 IU / kg vitamin D 3,respectively. Fowls were fed for 6 weeks in cages. The tissues( thymus,duodenum,jejunum,ileum,cecum and bursa of Fabricius) were collected for the analysis of relative expression levels of IL-2 and IL-18 genes. The results showed as follows: 6 400 IU / kg vitamin D 3 up-regulated the relative expression level of IL-2 gene in tissues of duodenum,bursa of Fabricius and cecum,but no regulation was found in tissues of thymus,jejunum and ileum; 6 400 IU / kg vitamin D 3 upregulated the relative expression level of IL-18 gene in tissues of thymus,ileum,cecum and bursa of Fabricius of Silky fowls. The results indicate that dietary supplemental level of vitamin D 3 can affect the relative expression levels of IL-2 and IL-18 genes in Silky fowls,but the effects are differ in different tissues.
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