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当前位置: 首页 > 文章 > Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究 畜牧兽医学报 2014,45 (12) 2081-2087
Position: Home > Articles > Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs Chinese Journal of Animal and Veterinary Sciences 2014,45 (12) 2081-2087

Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究

作  者：
黎威;高鹏飞;石建中;张燕青;徐凯;刘宏;郭晓红;李步高;曹果清
单  位：
山西农业大学动物科技学院;大同市种猪场
关键词：
猪;Myf5;背最长肌;发育性表达
摘  要：
本研究旨在分析猪背最长肌中Myf5基因的发育性表达规律。采用实时荧光定量PCR和Western-blot技术检测了马身猪和大白猪从初生~180日龄7个阶段(初生、30、60、90、120、150和180d)背最长肌中Myf5基因mRNA和蛋白质发育性表达规律。结果表明,马身猪和大白猪背最长肌中Myf5基因mRNA相对表达量均表现出初生时最高,30日龄次之,60日龄时突然降低,此后基本维持稳定状态的规律。马身猪背最长肌中Myf5蛋白的相对表达量初生时最高,30日龄时最低,60~180日龄阶段,表达量呈现波动变化的不稳定趋势;在大白猪中,Myf5蛋白60日龄前的相对表达量极显著高于60日龄后的表达量(P<0.05),60日龄前各阶段间的表达量及60日龄后各阶段间的表达量差异不显著(P>0.05)。在同一日龄阶段,马身猪Myf5蛋白的表达量均显著或极显著地高于大白猪(60日龄阶段除外)。Myf5在mRNA和蛋白质水平上的表达与猪的遗传背景和发育阶段有关。
译  名：
Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs
作  者：
LI Wei;GAO Peng-fei;SHI Jian-zhong;ZHANG Yan-qing;XU Kai;LIU Hong;GUO Xiao-hong;LI Bu-gao;CAO Guo-qing;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;Datong Pig Breeding Farm;
关键词：
pig;;Myf5 gene;;longissimus dorsi;;developmental expression
摘  要：
The aim of this study was to investigate the expression patterns of Myf5 gene at the levels of mRNA and protein.The expression patterns of Myf5 gene at the levels of mRNA and protein in longissimus dorsi at 7developmental stages(1-,30-,60-,90-,120-,150-,and 180-dayold)of Mashen and Large White pigs were studied by quantitative real-time PCR and Westernblot.The results showed that the highest mRNA relative expression of Myf5 gene in longissimus dorsi was at 1-day-old,then at 30-day old both in Mashen and Large White pigs.The mRNA relative expression of Myf5 gene decreased sharply at 60-day-old,then keep stable lower levels afterwards.The expression of Myf5 protein in longissimus dorsi of Mashen pig at 1-day-old was the highest and the lowest at 30-day-old.During the stages from 60-to 180-day-old,the relative expression of Myf5 peotein in Mashen pig was in waves with trend of increase and decrease.In Large White pig,the relative expressions of Myf5 protein at the stages before 60-day-old were significantgreater than those at the stages after 60-day-old,wherease the expressions at detected stages before 60-day-old or after 60-day-old were not significant different.At the same stages,the relative expression of Myf5 protein in Mashen pig was always greater than that in Large White pig at all detectd stages apart from that at 60-day-old.The expression patterns of Myf5 at the levels of mRNA and protein were associated with the genetic background and the developmental stages of invididuals.

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