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茶树种质资源AFLP分析中DNA模板快速制备方法

作  者:
周李华;赵超艺;罗军武;黄建安;谭和平
单  位:
中国测试技术研究院;湖南农业大学园林园艺学院茶学系;广东省农业科学院茶叶研究所
关键词:
茶树;AFLP;DNA提取方法
摘  要:
采用氯仿:异戊醇一次抽提法提取茶树基因组DNA,再经纯化,结果表明,此法提取的DNA其OD260/OD280值在1.70~1.85之间,相对分子量≥23kb,且能被EcoRI酶解完全,并能获得清晰PCR图谱和AFLP指纹图谱。
译  名:
Speedy Method of Making Template DNA in Analysis of Tea Germplasm Based on AFLP Fingerprint
作  者:
ZHOU Li-hua1,4,ZHAO Chao-yi3,LUO Jun-wu1,*,HUANG Jian-an1,2,TAN He-ping4 (1.Department of Tea Science, College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China; 2.Key Laboratory of Tea Science, Ministry of Education, Changsha 410128, China; 3.Tea Research Institute, Guangdong Academy of Agricultural Sciences, Yingde 513042, China; 4.Institute of National Measurment and Testing Technology, Chengdu 610021, China)
关键词:
tea plant;AFLP(amplified fragment length polymorphism);DNA extraction
摘  要:
Genomic DNA was isolated once from tea plant by chloroform and isoamyl alcohol. The results showed that the values of OD260/OD280 for DNA samples obtained by this method are between 1.70 and 1.85 respectively with the molecular weight of DNA higher than 23kb. The DNAs have been completely digested by EcoR I. Amplified figures in the PCR, and AFLP fingerprinting figures are all obtained.

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