当前位置: 首页 > 文章 > 响应面法优化球孢白僵菌CXJ-1的发酵工艺条件 石河子大学学报(自然科学版) 2011,29 (6) 35-41
Position: Home > Articles > Optimizatiom of Fermentation Conditons for Beauveria bassiana CXJ-1Spore by Response Surface Methodology Journal of Shihezi University(Natural Science) 2011,29 (6) 35-41

响应面法优化球孢白僵菌CXJ-1的发酵工艺条件

作  者:
孙佰平;任娟;赵思峰;侯彩霞;王斌;刘启鹏
单  位:
石河子大学绿洲农作物病害防控重点实验室;新疆兵团化工绿色过程重点实验室
关键词:
球孢白僵菌;响应面优化;发酵工艺条件;产孢
摘  要:
为了提高球孢白僵菌CXJ-1(Beauveria bassiana CXJ-1)在液体发酵过程中分生孢子的产孢量,采用响应面法对球孢白僵菌CXJ-1产孢的发酵工艺条件进行优化。采用单因素实验确定了产孢发酵条件最适初始pH、发酵转速和发酵温度。通过Plackett-Burman实验设计和Box-Behnken实验设计响应面分析优化,确定了球孢白僵菌CXJ-1产孢最佳发酵工艺参数为:温度28.07℃,转速198.95r/min,初始pH 6.10,预测孢子浓度可达9.5×108个/mL,在优化发酵条件下进行验证实验,孢子浓度达到9.7×108个/mL,表明验证值和预测值有较高的吻合度。
译  名:
Optimizatiom of Fermentation Conditons for Beauveria bassiana CXJ-1Spore by Response Surface Methodology
作  者:
SUN Baiping1,2,REN Juan2,ZHAO Sifeng2,HOU Caixia2,WANG Bin2,LIU Qipeng2 (1 Xinjiang Bingtuan Key Laboratory for Green Processing of Chemical Engineering Shihezi 832003,China; 2 The key laboratory of Prevention and Control for Oasis Crop Disease,Shihezi University,Shhezi 832003,China)
关键词:
Beauveria bassiana;response surface optimization;fermentation conditons;sporulation
摘  要:
Response surface methodology was used to optimize fermentation conditons in order to improve spore production of Beauveria bassiana CXJ-1.The optimum fermentation temperature,rotation speed and pH were obtained with one-factor-at-time.Through Plackett-Burman experimental design and Box-Behnken experimental design for response surface optimization,the optimal conditions for spore production of Beauveria bassiana CXJ-1 were also determined as follows:temperature 28.07 ℃,rotation speed 198.95 r/min and the pH value 6.10.Under these conditions,the predicted value of the concentration of spores was 9.5×108 spores/mL and the verified value was 9.7×108 spores/mL,the fermentation validation showed that the experimental value have high inosculating degree to model prediction.

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