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当前位置: 首页 > 文章 > 不同培养条件对牛核移植胚胎体外发育的影响 安徽农业科学 2008,36 (18) 186-187+269
Position: Home > Articles > Effect of the Different Cultivation Condiontions on Invitro Development of Bovine Embryos Journal of Anhui Agricultural Sciences 2008,36 (18) 186-187+269

不同培养条件对牛核移植胚胎体外发育的影响

作  者：
史文升;村上正夫;柏学进;王文明;刘玮;董雅娟
单  位：
青岛农业大学动物胚胎工程中心
关键词：
牛;核移植;胚胎;体外发育
摘  要：
[目的]进一步优化牛核移植胚胎的体外培养体系。[方法]在培养液中添加谷胱甘肽和胚胎培养的0~3 d采用5%O2浓度,在不同培养条件下对牛核移植重构胚胎进行体外培养,测定卵裂率8、细胞发育率、桑椹胚率以及囊胚发育率。[结果]结果表明,试验组B的8细胞发育率(38.3%)、桑椹胚率(17.0%)和囊胚率(8.5%)与对照组A(17.9%、1.3%、1.3%)差异显著(P<0.05)。试验组B和试验组C的桑椹胚率(17.0%、11.8%)、囊胚率(8.5%、7.8%)与对照组A(1.3%、1.3%)均差异显著(P<0.05)。[结论]在培养液中添加谷胱甘肽和胚胎培养的0~3d采用低O2浓度(5%O2),能够提高牛核移植胚胎的体外发育率。
译  名：
Effect of the Different Cultivation Condiontions on Invitro Development of Bovine Embryos
作  者：
SHI Wen-sheng et al(Animal Embryo Engineering Center,Qingdao Agricultural University,Qingdao,Shandong 266109)
关键词：
Bovine;Nuclear transplant;Embryo;Invitro development
摘  要：
[Objective]The aim of the research was to optimize the invitro development system of bovine nuclear transplant(NT) embryos.[Method]Appending the glutathione in the culture medium and reducing O2(5%) concentration during the first 3 days,bovine nuclear transplant(NT) embryos were examined after being cultured under different conditions.Cleavage rate,8 cell growth rate,morula rate as well as pouch embryo growth rate was determined respectively.[Result]The results indicated that 8 cell growth rate(38.3%),morula rate(17.0%) and pouch embryo growth rate(8.5%) of group B and A(17.9%、1.3%、1.3%) had significant difference(P<0.05).Morula rate(17.0%,11.8%) and pouch embryo growth rate(8.5%,7.8%) of group B and C were significantly higher(P<0.05) than that of group A.[Conclusion] Appending the glutathione that of the culture medium and reducing O2(5% O2) concentration during the first 3 days could increase the invitro development rate of bovine NT embryos.

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