当前位置: 首页 > 文章 > 不同线虫在黑松体内的扩展速度与致病性 南京林业大学学报(自然科学版) 2007,31 (1) 9-13
Position: Home > Articles > Research on the Relationship Between the Pathogenicity and the Distribution Velocity of the Nematode in seedling of Pinus thunbergii Journal of Nanjing Forestry University(Natural Sciences Edition) 2007,31 (1) 9-13

不同线虫在黑松体内的扩展速度与致病性

作  者:
金钢;叶建仁
单  位:
南京林业大学森林资源与环境学院
关键词:
松材线虫;拟松材线虫;黑松;致病力
摘  要:
分别用强、弱致病力松材线虫和无致病力拟松材线虫接种2年生黑松苗。观察结果表明:强致病力松材线虫在黑松体内扩散速度较快,4 d针叶开始发黄,7d松苗新梢开始萎蔫下垂,10 d松苗萎蔫,线虫在松苗体内大量繁殖,超过接种时的线虫数;弱致病力松材线虫在黑松体内扩散速度较慢,7 d针叶出现发黄的迹象,15 d新梢开始萎蔫,25 d松苗整株萎蔫,线虫数量开始增加,超过接种时的线虫数;无致病力拟松材线虫接种后7 d仅在树梢分离到少量线虫,梢部部分针叶发黄,之后线虫基本未繁殖,25 d松苗体内已分离不到线虫,梢部针叶症状未加剧,松苗生长与对照类似。因此,黑松感染线虫后,是否发病与线虫能否在松苗体内存活有关,发病速度快慢与线虫在松苗体内扩散速度及能否大量繁殖有直接关系。
译  名:
Research on the Relationship Between the Pathogenicity and the Distribution Velocity of the Nematode in seedling of Pinus thunbergii
作  者:
JIN Gang,YE Jian-ren*(College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China)
关键词:
Bursaphelenchus xylophilus;Bursaphelenchus mucronatus;Pinus thunbergii;Pathogenicity
摘  要:
The experiment adopted peel-grafting method to inoculate the 2a Pinus thunbergii seedling with strong and weak pathogenicity Bursaphelenchus xylophilus as well as non-pathogenicity Bursaphelenchus mucronatus.The observation results showed that the strong pathogenicity Bursaphelenchus xylophilus spreaded more quickly inside the Pinus thunbergii.The needle begins to turn yellow in 4d.The new tip of pine seedling begin to wilt in 7d,and died completely in 10d.The amount of the nematode was much more than that of inoculated because the nematode reproduced in a great deal inside of the seedling.The weak pathogenicity Bursaphelenchus xylophilus spreaded relatively slowly inside the Pinus thunbergii.The needle turned yellow in 7d.The new tip begins to wilt in 15d.The seedling died in 25d.The amount of nematodes began to increase.After non-pathogenicity Bursaphelenchus mucronatus had been inoculated,only small amount of nematodes from the tree top was separated in 7d.Some needles on the tip turned yellow.The nematode didn't reproduce basically. The nematode can not be separated from the seedling in 25d.The needle symptom on the tip hasn't strengthened which doesn't lead to the death of the new seedlings.Therefore,after the Pinus thunbergii was infected with nematode,whether it wound come down with disease was closely related to the nematode survival inside the pine seedling.The symptom of the disease has direct relations to the nematode spread speed inside the pine seedling as well as whether it can reproduce considerably.

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