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当前位置: 首页 > 文章 > 中国奶牛PrP基因型分析 中国畜牧兽医 2009,36 (3) 110-112
Position: Home > Articles > Analysis of Genotype of PrP Gene in China Cow China Animal Husbandry & Veterinary Medicine 2009,36 (3) 110-112

中国奶牛PrP基因型分析

作  者：
张太翔;田国华;田国宁;张金玲;韩亮;张丽萍
单  位：
潍坊出入境检验检疫局
关键词：
黑白花奶牛;朊病毒基因型;多态性分析
摘  要：
根据已报道奶牛朊蛋白(PrP)基因序列设计引物,采用PCR法扩增了28头黑白花奶牛的PrP基因。序列测定及分析结果表明,所克隆的奶牛PrP基因片段为677bp,编码225个氨基酸的前体蛋白。对其136、154、171位点氨基酸多态性分析结果发现,对TSE中度易感的ARQ型占检测奶牛的82.9%,对TSE抗性的ARR型占7.1%,对TSE高度易感的VRQ型占10%。这说明中国奶牛的基因型对TSE中度易感,为防制TSE的发生提供基础数据。
译  名：
Analysis of Genotype of PrP Gene in China Cow
作  者：
ZHANG Tai-xiang,TIAN Guo-hua,TIAN Guo-ning,ZHANG Jin-ling,HAN Liang,ZHANG Li-ping(Weifang Entry-Exit Inspection and Quarantine Bureau of People's Republic of China,Weifang 261041,China)
关键词：
black and white cow;genotype of PrP gene;polymorphism analysis
摘  要：
We got the PrP gene of 28 black and white cows by PCR using the primers designed according to the reported cow PrP gene.Sequence analysis showed the segment of cow PrP gene were 677 bp,coding 225 amino acids.The analysis of polymorphism of 136,154,171 amino acid site of prion protein revealed the genotype of ARQ associated with an intermediate susceptibility to TSE was present in 82.9% of cow examined,the genotype of ARR that associated with the resistance to TSE was present in 7.1% of cow examined,the genotype of VRQ associated with high susceptibility to TSE was present in 10% of cow examined.It suggested that the cow in our country was intermediate susceptibility to TSE,which could support the basic data to prevent and control the TSE in our country.

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