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Position: Home > Articles > Screening of Sweet Orange [Citrus. sinensis(L.) Osbeck] Mutants Resistant to Canker disease Using Ethyl Methane Sulphonate(EMS) Mutagenesis Techniques Hunan Agricultural Sciences 2015 (3) 1-4

利用EMS诱变获得抗溃疡病甜橙突变体的研究

作  者:
罗丽;李益;葛红娟;谢玉明;邓子牛
单  位:
湖南农业大学园艺园林学院国家柑橘改良中心长沙分中心
关键词:
柑橘溃疡病;甲基磺酸乙酯;粗提物;抗病突变体;离体筛选
摘  要:
研究以大红甜橙实生苗上胚轴为试材,采用甲基磺酸乙酯(EMS)对其节间茎段进行诱变处理,通过用不同浓度溃疡病菌粗提物进行离体筛选,以确定适宜的抗溃疡病选择压,并获得抗溃疡病突变体材料。结果表明,以1.8%的EMS溶液浸泡节间茎段2 h可获得"半致死"效应;溃疡病菌粗提物浓度为4%时为适宜的抗病选择压。对1 030个再生不定芽进行两次不连续离体筛选,再经离体接种溃疡病菌鉴定后获得35个抗性芽,成活率为3.4%。该试验初步获得抗溃疡病粗提物的大红甜橙突变体株系23株,为后续进一步研究提供材料基础。
译  名:
Screening of Sweet Orange [Citrus. sinensis(L.) Osbeck] Mutants Resistant to Canker disease Using Ethyl Methane Sulphonate(EMS) Mutagenesis Techniques
作  者:
LUO Li;LI Yi;GE Hong-juan;XIE Yu-ming;DENG Zi-niu;College of Horticulture and Landscape, Hunan Agricultural University,National Center of Citrus Improvement,Changsha Subcenter;
关键词:
Xanthomonas citri subsp.citri;;ethyl methylanesulphonate(EMS);;crude extracts;;resistant mutants;;in vitro screening
摘  要:
Xanthomonas citri subsp. citri is the causal agent of citrus canker. Citrus canker disease has been one of the serious diseases in the south of China, and caused serious damages. Sweet orange [Citrus. sinensis(L.) Osbeck] is the most important citrus species widely cultivated in the world, but all the sweet orange cultivars are highly susceptible. The essential and effective solution is to breed a new commercial citrus cultivars with good quality, high yield and high resistant to canker disease. The epicotyl internodal stem segments of sweet orange seedling were treated with ethyl methylanesulphonate(EMS) solution and screened by the Xcc-crude extracts produced by Xanthomonas citri subsp. citri, via a multi-step screen method. The results showed that the stem segments were treated with 1.8%(v/v) of EMS for 2h which resulted in the effect of the "semi lethal dose", and the appropriate selection pressure was that the stem segments were cultured in medium supplemented with 4%(v/v) of Xcc-crude extracts. There were 35 regenerated buds survival in 1030 after mutation and twice discontinuous screening the survival rate was 3.4%, and of which 23 strains were survived after grafted on the root stock of Poncirus trifoliata(L.) Raf.

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