Position: Home > Articles > Establishment of detection method for viable cells of Acidovorax avenae subsp. citrulli with PMA-PCR
China Plant Protection
2015,35
(9)
12-15
西瓜细菌性果斑病病菌活细胞PMA-PCR检测方法的建立
作 者：
赵璟源;胡佳续;张莹;刘鹏;唐芳;廖芳
单 位：
天津出入境检验检疫局
关键词：
西瓜细菌性果斑病病菌;叠氮溴化丙锭;聚合酶链式反应;活细胞检测
摘 要：
利用叠氮溴化丙锭与聚合酶链式反应结合(PMA-PCR),建立了西瓜细菌性果斑病病菌(Acidovorax avenae subsp.citrulli)活细胞检测方法。实验结果表明,当PMA浓度为0.5 mg/m L时,对活细胞DNA的PCR扩增无影响,最低检测浓度为1×104cfu/m L。当样品中死亡细胞浓度低于107 cfu/m L时,PMA可抑制死亡细胞DNA的PCR扩增;当死亡细胞浓度为1×107cfu/m L时,PMA失去效果。该方法适用于低浓度西瓜细菌性果斑病病菌活细胞的检测。
译 名：
Establishment of detection method for viable cells of Acidovorax avenae subsp. citrulli with PMA-PCR
作 者：
Zhao Jingyuan;Hu Jiaxu;Zhang Ying;Liu Peng;Tang Fang;Liao Fang;Tianjin Entry-Exit Inspection and Quarantine Bureau;
关键词：
Acidovorax avenae subsp.citrulli;;propidiummonoazide;;PCR;;viable cells detection
摘 要：
Detection method for viable cells of Acidovorax avenae subsp. citrulli(Aac) was established with propidiummonoazide(PMA) and PCR. The results showed that PCR amplification of DNA from viable cells was not inhibited by PMA with concentration of 0.5 mg/m L, and minimum detectable concentration of Aac was 104cfu/m L. PCR amplification of DNA from dead cells was inhibited by PMA with suspensions of dead Aac containing less than 107cfu/m L.PMA treatment was ineffective with suspensions of dead Aac containing at 1 ×107cfu/m L. The method was applicable for detection of low concentration of living cells of Aac.
导出引用
计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
