The genetic diversity of farmed tapertail anchovy Coilia nasus and Coilia nasus taihuensis by mitochondrial D-loop genes analysis

XU Gang-chun1,WEI Guang-lian2,LI Jian-lin1,XU Pao1,ZHANG Cheng-xiang3,GU Ruo-bo1(1.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;2.College of Fisheries,Nanjing Agriculture University,Wuxi 214081,China;3.Extension Station of Aquiculture Technology of Jiangyin,Jiangyin 214431,China)

farmed Coilia nasus;Coilia nasus taihuensis;D-loop region;genetic diversity

Genetic diversity of two genetic-ecological populations in farmed tapertail anchovy Coilia nasus and Coilia nasus taihuensis were assayed by D-loop sequence of mtDNA as a molecular maker.The complete mitochondrial D-loop gene sequences of farmed Coilia nasus was shown to be ranged from 1 210 bp to 1 252 bp and Coilia nasus taihuensis to be varied from 1 252 to 1 290 bp in length.Total of 35 variation sites including 14 singleton polymorphic sites and 21 parsimony informative polymorphic sites were defined in the 19 individuals sampled,in which 12 haplotypes were detected.The haplotype diversity(H) was found to be 0.924,nucleotide diversity(π) 0.0099,the average nucleotide difference(K) 4.154 and the average genetic distance 0.0148.There were a little higher genetic parameters and heredity diversities in the farmed Coilia nasus than those in Coilia nasus taihuensis.It was found that there was significantly smaller average genetic distance(0.0148) between farmed Coilia nasus and Coilia nasus taihuensis than that(0.0528) between each species of farmed Coilia nasus and Coilia nasus taihuensis and Coilia grayii(0.0537).The phylogeny tree also showed that a monophyletic group was comprised of the farmed Coilia nasus and Coilia nasus taihuensis,on differentiation of species or subspecies.