作 者：
黄放;高卫东;张海波;祝燕;裴昊斐;方娇阳;李庆梅
单 位：
中国林业科学研究院林业研究所国家林业和草原局林木培育重点实验室林木遗传育种国家重点实验室
摘 要：
[目的]以侧柏(Platycladus orientalis(L.) Franco)种子为试验材料,通过对不同浓度水杨酸(SA)浸种处理下侧柏种子萌发指标、幼苗形态和生理指标的定量分析,研究SA对侧柏种子萌发特性和幼苗生长的影响,筛选出最佳的SA浸种浓度,为侧柏播种育苗的生产实践提供参考。[方法]设置0.5、1.0、1.5、2.0、3.0 mmol·L~(-1 )5个SA浓度,对侧柏种子进行浸种,蒸馏水浸种为对照,测定各处理种子发芽指标、形态指标、幼苗的抗氧化酶活性和丙二醛(MDA)含量指标。[结果]SA浸种可以显著提高侧柏种子的发芽率和发芽指数,1.5 mmol·L~(-1)处理的发芽率最高,为96.3%,比对照提高9.3%,3.0 mmol·L~(-1)处理的发芽指数最高,比对照提高26.5%;低浓度SA(≤1.0 mmol·L~(-1))有促进地上部分生长、抑制地下部分生长的效果;高浓度SA(≥1.5 mmol·L~(-1))则抑制地上部分生长,促进地下部分生长;超氧化物歧化酶(SOD)活性在SA浓度1.5 mmol·L~(-1)时最高,比对照提高69.0%;过氧化氢酶(CAT)活性随SA浓度的增大而升高,在1.5、2.0、3.0 mmol·L~(-1)处理下均高于对照,分别增加33.9%、10.7%、19.9%;随SA处理浓度的增大,MDA含量逐渐降低,但差异不明显,可能和SA促进抗氧化酶活性,减少膜脂过氧化,种子本身质量良好,且外界无逆境胁迫有关。[结论] 1.5～3.0 mmol·L~(-1 )SA处理对提高侧柏种子发芽率和幼苗SOD、CAT活性及促进幼苗地下部分生长效果较佳,但各浓度间的处理效果差异不显著。综合考虑生产成本等因素,建议生产实践中侧柏种子播种育苗时采用1.5 mmol·L~(-1 )SA浸种24 h。
译 名：
Effect of Salicylic Acid Soaking on Seed Germination and Seedling Physiological Characteristics of Platycladus orientalis
作 者：
HUANG Fang;GAO Wei-dong;ZHANG Hai-bo;ZHU Yan;PEI Hao-fei;FANG Jiao-yang;LI Qing-mei;State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry and grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry;
单 位：
HUANG Fang%GAO Wei-dong%ZHANG Hai-bo%ZHU Yan%PEI Hao-fei%FANG Jiao-yang%LI Qing-mei%State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry and grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry
关键词：
Salicylic acid;;Platycladus orientalis;;antioxidant system;;enzyme activity;;germination rate
摘 要：
[Objective] To investigate the effect of salicylic acid(SA) on seed germination, seedling morphological and physiological index of Platycladus orientalis and to select the best concentration of SA. [Method] Soaking application of SA was used to process P. orientalis seed with 5 concentrations(0.5, 1.0, 1.5, 2.0 and 3.0 mmol·L~(-1)), and the seeds soaked in distilled water as a control. The seed germination index, morphological index, antioxidant enzyme activity and the content of MDA were measured in the end of germination. [Result] SA soaking significantly improved the germination rate and germination index of P. orientalis, 1.5 mmol·L~(-1) treatment manifested the highest rate of 96.3%, which was 9.3% higher than that of the control. The highest germination index was 3.0 mmol·L~(-1), which was 26.5% higher than that of the control. Low concentration SA(≤1.0 mmol·L~(-1)) promoted the seedling growth of aerial part and inhibited the underground part. High concentration SA(≥1.5 mmol·L~(-1)) inhibited the seedling growth of aerial part and promoted the underground part. The activity of SOD was the highest in 1.5 mmol·L~(-1) SA, which increased by 69.0%. The CAT activity with 1.5 mmol·L~(-1), 2.0 mmol·L~(-1) and 3.0 mmol·L~(-1) were higher than that of the control, which increased by 33.9%, 10.7% and 19.9%. The MDA content decreased gradually, but the difference was not significant among treatments. It is possible that SA could improve the activities of antioxidant enzymes and reduce the membrane lipids. Besides, the seeds themselves were in good quality, and the external environment was not related to stress. [Conclusion] 1.5-3.0 mmol·L~(-1) SA was better to increase the germination rate, SOD and CAT activities of seedlings, improve the seedling growth of underground part. And there was no significant difference among treatments. For reducing the production costs, 1.5 mmol·L~(-1) SA is considered in applications.
