中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 松树土壤根际微生物溶磷基因的克隆与表达 湖北农业科学 2017,56 (20) 3952-3954
Position: Home > Articles > Cloning and Expression of the Phosphate Soluble Gene of the Rhizosphere Microorganism Hubei Agricultural Sciences 2017,56 (20) 3952-3954

松树土壤根际微生物溶磷基因的克隆与表达

作  者：
吴雪;金辉;唐健;罗贞贞
单  位：
广西产品质量检验研究院
关键词：
根际微生物;溶磷;GabY基因
摘  要：
探究土壤根际微生物溶磷基因对难溶性矿物质磷的溶解作用。利用从土壤中提取宏基因组,克隆了Gab Y基因,并构建了表达载体,研究了该基因在大肠杆菌DH5α的表达对难溶矿物质磷酸Ca_3(PO_4)_2的溶解作用。结果表明,构建的Gab Y表达载体成功表达;对照组产酸能力较试验组弱,且对照组溶磷效率要低于试验组,直接从植物根际土壤微生物宏基因组中筛选溶磷基因对矿物质无机磷的溶解的研究可行。
译  名：
Cloning and Expression of the Phosphate Soluble Gene of the Rhizosphere Microorganism
作  者：
WU Xue;JIN Hui;TANG Jian;LUO Zhen-zhen;Guangxi Zhuang Autonomous Region Forestry Research Institute;Guangxi Zhuang Autonomous Region Institute of Product Quality Inspection;Key Laboratory of Fast-growing Timber Breeding and Cultivation of Forestry in Central South China of Ministry of National Forestry;Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation;
单  位：
Guangxi Zhuang Autonomous Region Forestry Research Institute%Guangxi Zhuang Autonomous Region Institute of Product Quality Inspection%Key Laboratory of Fast-growing Timber Breeding and Cultivation of Forestry in Central South China of Ministry of National Forestry%Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation
关键词：
rhizosphere microorganisms;;soluble phosphorus;;GabY gene
摘  要：
To study the dissolving function of Gab Y gene on mineral inorganic phosphorus. The macro genomic DNA from soil was extracted,Gab Y gene was cloned, and then integrated into expression vector p ETDuet-1,induced the integrated vector expression in DH5α to research the dissolving function on Ca_3(PO_4)_2. The results showed Gab Y gene expression was successfully induced,and was able to promote the dissolving efficiency of inorganic phosphorus. Directly selected the mineral inorganic phosphorus soluble gene from soil macro genomic DNA is a viable method.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46