Position: Home > Articles > Sequencing of Elongation Factor 2 from Lupinus luteus
Scientia Agricultura Sinica
2002,35
(1)
102-105
黄羽扇豆翻译延伸因子2的序列分析
作 者：
陈建中;章镇;戴剑;R.Slomski
单 位：
江苏省农业科学院粮食作物研究所;波兹南农业大学生化和生物技术系;南京农业大学园艺学院
关键词：
黄羽扇豆;延伸因子2;亚克隆;序列分析
摘 要：
利用Sanger双脱氧链终止法对黄羽扇豆（Lupinusluteus）翻译延伸因子2（EF2）的全长cDNA克隆进行了序列分析。该cDNA长度为2794bp,其中包括5’末端的80bp非翻译区,3’末端185bp非翻译区和2529bp长编码序列,3’末端为一18bp poly（A）尾。与其它GTP结合蛋白比较其编译的843aa（氨基酸）序列,发现它们具有显著的同源性;黄羽扇豆EF2与甜菜EF2的氨基酸序列90％相同。其差异主要存在于序列的中部;而与肽基tRNA和核糖体作用部位、与GTP结合部位和GTP酶活性有关的部位具有很高的保守性。黄羽扇豆EF2第700个氨基酸残基组氨酸是白喉毒素的ADP-核糖基化部位。
译 名：
Sequencing of Elongation Factor 2 from Lupinus luteus
作 者：
CHEN Jian-zhong ,DAI Jian ,ZHANG Zhen ,R. Slomski( College of Horticulture , Nanjing Agricultural University , Nanjing 210095 ; Institute of Food Crops ,Jiangsu Academy of Agricultural Sciences , Nanjing 210014 ; Department of Biochemistryand Biotechnol
关键词：
Lupinus luteus ; EF2; Subcloning; Sequencing
摘 要：
A full-length cDNA clone encoding Lupinus luteus elongation factor 2 (EF2) involved in the elongation step of protein synthesis was sequenced. The cDNA insert contains a 80-bp 5'-untranslated region,a 2529-bp long coding sequence and a 185-bp 3'-untranslated region. The deduced amino acid sequence (843 residues) from this 2794 bp nucleotide sequence shares 90% homology with that from Beta vulgaris. The sequence analysis supports that diphthamide {2-[3-carboxyamido-3-(trimethylammonio) propyl] histidine( , the site of ADP-ribosylation by diphtheria toxin (DT), is produced by post-translational modification of a histidine residue in the primary translational product. Sequence comparision with other EF2s and GTP-binding protein typa shows that the domains, which are likely to be involved in GTP binding and GTPase activity, and interaction with ribosome and toxins,are highly conserved.
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