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当前位置: 首页 > 文章 > PCR扩增法检测江苏省5大淡水湖泊产毒微囊藻的空间分布 农业环境科学学报 2012 (11) 2215-2222
Position: Home > Articles > Spatial Distribution of Toxic Microcystis Detected by PCR Amplification in Five Freshwater Lakes of Jiangsu Province,China Journal of Agro-Environment Science 2012 (11) 2215-2222

PCR扩增法检测江苏省5大淡水湖泊产毒微囊藻的空间分布

作  者：
李大命;阳振;于洋;唐晟凯;张彤晴
单  位：
中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室;中国科学院研究生院;江苏省淡水水产研究所
关键词：
蓝藻水华;微囊藻毒素合成酶基因;富营养化指数;多重PCR
摘  要：
微囊藻毒素(Microcystin,MC)的产生受微囊藻毒素合成酶基因簇(microcystin biosynthesis gene,mcy)调控,常用PCR扩增mcy基因检测产毒微囊藻。采集江苏省5大淡水湖泊——太湖、滆湖、高宝-邵伯湖、洪泽湖和骆马湖的水样,测定水体营养盐浓度和叶绿素a浓度,根据叶绿素a浓度计算5个湖泊的富营养化指数(Trophic state index,TSI),同时应用单一和多重PCR扩增mcy基因。结果表明,太湖和滆湖处于富营养和超富营养化水平,洪泽湖和骆马湖处于中营养和富营养化水平,高宝-邵伯湖处于寡营养水平。太湖、滆湖、洪泽湖和骆马湖的所有水样均检出mcy基因,4个湖泊水体受到微囊藻毒素的潜在威胁,高宝-邵伯湖没有检测出mcy基因的存在,尚未受微囊藻毒素的污染。
译  名：
Spatial Distribution of Toxic Microcystis Detected by PCR Amplification in Five Freshwater Lakes of Jiangsu Province,China
作  者：
LI Da-ming1,2,YANG Zhen2,YU Yang2,TANG Sheng-kai1,ZHANG Tong-qing1,ZHOU Gang1,PAN Jian-lin1(1.Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China;2.State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,Nanjing 210008,China)
关键词：
cyanobacterial bloom;microcystin synthetase gene;trophic state index;multiple PCR;
摘  要：
Blooms of toxic Microcystis have became increasing common in eutrophic freshewater lakes of the world.The most prominent toxin produced by Microcystis is the hepatotoxin microcystin(MC),a cyclic heptapeptide which pose significant threat to human and animal health.MC are synthesized by the enzyme complex microcystin synthetase,which includes a nonribosomal peptide synthetase and a polyketide synthase,both encode by microcystin biosynthesis genes(mcy).Therefore,mcy genes are the most common gene markers used for detection of toxic Microcystis in natural environments by means of PCR amplification.In this study,water samples were collected from different lake area of five large shallow freshwater lakes:Lake Tai,Lake Ge,Lake Gaobao-Shaobo,Lake Hongze and Lake Luoma in summer season of 2011.For all 32 water samples,the concentrations of Chlorophyll a(Chl-a)and nutrients were measured.Furthermore,trophic state index(TSI)was calculated from Chl-a concentrations.Meanwhile single and multiplex PCR amplification were used to detect whether the presence of mcy genes or not in water samples.TSI values demonstrated that Lake Tai and Lake Ge were in eutrophic and hypereutrophic state,Lake Hongze and Lake Luoma were in mesotrophic and eutrophic state and Lake Gaobao-Shaobo was in oligotrophic state.The results from PCR analysis showed that there were the presence of PCR products of partial mcyA,mcyD and mcyG genes in water samples from Lake Tai,Lake Ge and Lake Hongze,only mcyA PCR products were detected in Lake Luoma,but no any PCR products were found in Lake GaobaoShaobo water samples.In conclusion,Lake Tai,Lake Ge,Lake Hongze and Lake Luoma are potentially contaminated by microcystin,however,no microcystin are present in the Lake Gaobao-Shaobo now.

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