Construction of glycan-functionalized nanoparticles and its application in the detection of sialic acid-binding protein

QI Xiaoxiao;LI Xuebing;ZHOU Xuzheng;ZHANG Jiyu;ZHANG Zhenxing;WU Peixing;Lanzhou Institute of Husbandry and Pharmaceutical,Chinese Academy of Agricultural Sciences;Institute of Microbiology/Key Laboratory of Pathogenic Microbiology and Immunology,Chinese Academy of Sciences;

sialic acid-binding protein;;fluorescence resonance energy transfer;;quantum dots;;gold nanoparticles;;wheat germ agglutinin

For the rapid and simple detection of influenza viruses,a novel strategy based on fluorescence resonance energy transfer(FRET) was developed.By using ligand exchange method,glycan-functionalized quantum dots(gQDs) and glycan-functionalized gold nanoparticles(gAuNPs) were synthesized,respectively.The results showed that:The characterization of gQDs and gAuNPs revealed that they could be used as an effective FRET donor-acceptor pair because of their excellent optical properties,high aqueous solubility and stability;Based on these two glycan-functionalized nanomaterials,a FRET biosensor system was developed.Wheat germ agglutinin,which is a sialic acid-binding protein(SABP),was detected within 5 minutes with detection limit of 4 nmol/L.Because influenza viruses use their envelope SABP,hemagglutinin,to specificly recognize and attach to sailic acids displayed on the host cell surface.The method developed in this study would provide bases to develop gQDs and gAuNPs-based FRET systems for rapid and simple influenza virus detection.