中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 3种白鲑线粒体细胞色素b和16S rRNA基因片段序列分析 中国水产科学 2007,14 (1) 8-14
Position: Home > Articles > Sequence analysis of partial cytochrome b and 16S rRNA genes of three Coregonus species Journal of Fishery Sciences of China 2007,14 (1) 8-14

3种白鲑线粒体细胞色素b和16S rRNA基因片段序列分析

作  者：
张俊丽;高天翔;韩志强;郭焱;董崇智
单  位：
中国海洋大学海水养殖教育部重点实验室;中国水产科学研究院黑龙江水产研究所;新疆维吾尔自治区水产研究所
关键词：
乌苏里白鲑;贝加尔凹目白鲑;高白鲑;细胞色素b;16S rRNA
摘  要：
对黑龙江水域的乌苏里白鲑(Coregonus ussuriensis)和由俄罗斯引种的贝加尔凹目白鲑(C.autumnalis migrato-rius)和高白鲑(C.peled)的线粒体细胞色素b(Cytb)和16S rRNA基因片段进行了扩增和序列测定,分析比较了3种白鲑间的序列差异。在402 bp的Cytb基因片段中,3个乌苏里白鲑个体中出现了2种单倍型,种内个体间有2个碱基的差异,而贝加尔凹目白鲑和高白鲑的个体各出现1种单倍型;在577 bp的16S rRNA基因片段中,3个乌苏里白鲑个体出现了2种单倍型,种内个体间存在1个碱基的差异,而贝加尔凹目白鲑和高白鲑2个种的序列完全相同。研究结果表明,Cytb基因适用于白鲑的分子系统发育研究。基于Cytb基因片段序列,利用Kimura-2模型构建的NJ树表明:乌苏里白鲑与贝加尔凹目白鲑亲缘关系较近;乌苏里白鲑与贝加尔凹目白鲑和高白鲑的分歧时间分别约为62.5万年和152万年,在更新世。[中国水产科学,2007,14(1):8-14]
译  名：
Sequence analysis of partial cytochrome b and 16S rRNA genes of three Coregonus species
作  者：
ZHANG Jun-li~(1),GAO Tian-xiang~(1),HAN Zhi-qiang~(1),GUO Yan~(2),DONG Chong-zhi~(3)(1.The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China;2.Xinjiang Weiwur Autonomous Region Fishery Institute,Wulumuqi 830000,China;3.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)
关键词：
Coregonus ussuriensis;C.autumnalis migratorius;C.peled;cytochrome b;16S rRNA
摘  要：
To evaluate the genetic variance of three Coregonus fish of Coregonus ussuriensis,C.autumnalis migratorius and C.peled,partial sequence of cytochrome b and 16S rRNA genes from three individuals of each Coregonus species were amplified and sequenced.The sequence data of partial cytochrome b gene(402 bp) showed that there were two haplotypes(C.u1 and C.u2) in the three individuals of C.ussuriensis.There was only one haplotype found in C.autumnalis migratorius(C.am) and C.peled(C.p1),respectively.Of the 577 nucleotides of partial 16S rRNA gene,no interspecific sequence variations were observed between C.autumnalis migratorius and C.peled and two haplotypes were observed in the three C.ussuriensis individuals.This suggests that the evolution rate of cytochrome b was faster than that of 16S rRNA in Coregonus.Based on sequences of partial cytochrome b gene,a phylogenetic tree was constructed by NJ method.The phylogenetic tree showed that C.ussuriensis and C.autumnalis migratorius was closely related.The estimated divergence time between C.ussuriensis and C.autumnalis migratorius was about 0.625 million years ago,while it was about 1.52 million years ago between C.ussuriensis and C.peled.The divergence of the three Coregonus species might had occurred in Pleistocene.[Journal of Fishery Sciences of China,2007,14(1):8-14]

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46