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Position: Home > Articles > Identification of Liriodendron tulipifera,Liriodendron chinense and Hybrid Liriodendron Using Species-Specific SSR Markers Scientia Silvae Sinicae 2010,46 (1) 39-42

鹅掌楸属种及杂种的SSR分子鉴定

作  者:
张红莲;李火根;胥猛;冯源恒
单  位:
南京林业大学林木遗传与生物技术省部共建教育部重点实验室
关键词:
鹅掌楸;北美鹅掌楸;杂交鹅掌楸;SSR分子标记;特异性鉴定
摘  要:
以不同种源的鹅掌楸、北美鹅掌楸和不同组合的杂交鹅掌楸为试验材料,对176对SSR引物进行初筛、复筛,最后筛选出1对物种特异性SSR引物。该引物在鹅掌楸群体中特异性扩增出190bp的产物,在北美鹅掌楸群体中特异性扩增出180bp的产物,在杂交鹅掌楸群体中特异性扩增出180bp和190bp2种产物。该引物对鹅掌楸种间鉴别准确率达100%。
译  名:
Identification of Liriodendron tulipifera,Liriodendron chinense and Hybrid Liriodendron Using Species-Specific SSR Markers
作  者:
Zhang Honglian Li Huogen Xu Meng Feng Yuanheng (Key Laboratory of Genetics & Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037)
关键词:
Liriodendron tulipifera; Liriodendron chinense; hybrid Liriodendron; SSR marker; specific identification
摘  要:
This paper reported that species-specific SSR markers were used to identify Liriodendron tulipifera,L.chinense and hybrid Liriodendron.Overall,90 genotypes were taken as DNA amplification templates,of which 30 came from 20 provenances of L.chinense,30 from 5 provenances of L.tulipifera,and 30 from 30 mating combinations of hybrid Liriodendron.Upon two round amplifying and screening,one species-specific pair primers for Liriodendron were screened from 176 pair primers.With the pair primers,a 190 bp fragment from L.chinense,180 bp fragment from L.tulipifera,and a 190 bp and a 180 bp fragment from hybrid Liriodendron were respectively amplified.To testify the veracity of these pair primers,10 individuals from L.chinense,5 from L.tulipifera,and 10 from hybrid Liriodendron were used as amplification templates,and the results showed 100% veracity for species identification.Therefore,the development of the pair primers provided an effective,stable and reliable method for species-specific identification in Liriodendron at the molecular level.

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